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Review ArticleArticle

Association of the Intergenic Single-Nucleotide Polymorphism rs10865331 (2p15) with Ankylosing Spondylitis in a Spanish Population

ALEJANDRA SÁNCHEZ, MAGDALENA SZCZYPIORSKA, XAVIER JUANOLA, NEREA BARTOLOMÉ, JORDI GRATACÓS, PEDRO ZARCO, EDUARDO COLLANTES, MARTA ARTIEDA, ANTONIO MARTÍNEZ, DIEGO TEJEDOR and JUAN MULERO
The Journal of Rheumatology November 2010, 37 (11) 2345-2347; DOI: https://doi.org/10.3899/jrheum.100211
ALEJANDRA SÁNCHEZ
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  • For correspondence: malejandra_sanchez@yahoo.es
MAGDALENA SZCZYPIORSKA
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XAVIER JUANOLA
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NEREA BARTOLOMÉ
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JORDI GRATACÓS
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PEDRO ZARCO
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EDUARDO COLLANTES
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MARTA ARTIEDA
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ANTONIO MARTÍNEZ
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DIEGO TEJEDOR
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JUAN MULERO
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Abstract

Objective. A recent genome-wide association study has identified 2 single-nucleotide polymorphisms (SNP) associated with ankylosing spondylitis (AS), rs10865331 (2p15) and rs2242944 (21q22). We assessed the association of these SNP with AS in a Spanish population.

Methods. Four hundred fifty-six patients with AS fulfilling the modified New York Criteria and 300 healthy donors were analyzed.

Result. SNP rs10865331 (allele A: p = 0.039; genotype: p = 0.016) was significantly associated with AS, while no association was found for rs2242944.

Conclusion. This is the first study that replicates in an independent cohort the association of the intergenic SNP rs10865331 with susceptibility to AS.

  • ANKYLOSING SPONDYLITIS
  • SINGLE-NUCLEOTIDE POLYMORPHISM
  • GENETIC STUDIES

Ankylosing spondylitis (AS) is a chronic inflammatory disease characterized by sacroiliitis, ankylosis of the spine, and enthesitis. Although the exact cause of AS is unknown, genetic factors have long been implicated in AS, beginning with the discovery of the association of HLA-B27 with the disease in the early 1970s1. However, although around 90% of white patients with AS are B27-positive, only < 5% of the approximately 8% of B27-positive individuals in the general population develop the disease. Over the last few years, the development of high-throughput microarray-based single-nucleotide polymorphism (SNP) genotyping techniques and genome-wide association studies (GWAS) have helped to highlight non-HLA genetic risk factors associated with AS, involving genes such as IL23R, ERAP1, CYP2D6, and IL1 gene complex, among others2,3,4,5,6. A recent GWAS confirmed the association of ERAP1, IL23R, and proinflammatory genes (IL1 complex and TNFR1) with AS and found a new association for 2 intergenic SNP: rs2242944, on chromosome 21q22, and rs10865331, on chromosome 2p157. It was the first time that an association for these intergenic regions with AS was reported, so we selected these 2 intergenic SNP for replication. Later, the same authors published extended results reporting an association with AS for the intergenic SNP rs4672495 and for SNP in the ANTXR2 gene8. Our purpose was to evaluate the association of the intergenic SNP rs2242944 and rs10865331 with AS in a Spanish population.

MATERIALS AND METHODS

Study population

The study population consisted of 456 patients with AS from the national registry of spondyloarthropathies of Spain (REGISPONSER)9, who fulfilled the modified New York criteria10, and 300 spondylarthritis-free individuals from the National DNA Bank of Spain. Both cases and controls were of Spanish white origin. The case cohort comprised 348 men and 108 women aged 51 ± 11 years. Controls were selected to have a similar sex ratio as cases, 225 men and 75 women, and to be over 50 years old, with a mean age of 55 ± 4.5 years. All patients gave written informed consent to participate, and the Ethics Committee of the Puerta de Hierro University Hospital centrally approved the study. The ethics approval for the control cohort was obtained from the Ethics Committee of the National DNA Bank of Spain.

SNP genotyping and HLA-B27 typing

The 2 SNP, rs10865331 and rs2242944, were genotyped using KASPar chemistry (KBioscience, Hertfordshire, UK), which is a competitive allele-specific polymerase chain reaction (PCR) SNP genotyping system using FRET quencher cassette oligonucleotides. HLA-B27 carriage was tested by conventional PCR11 using the amplification of exon 8 of p53 as an internal control12 (Table 1).

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Table 1.

Oligonucleotides used for genotyping of SNP rs10865331 and rs2242944 and for PCR detection of HLA-B27 and exon 8 of p53. The annealing temperature of the PCR was 62°C for HLA-B27 and p53 detection, and 57°C for rs10865331 and rs2242944 genotyping.

Statistical analysis

A test for deviation from Hardy-Weinberg equilibrium (HWE) was performed for each SNP in the control population using Helix Tree software v.6.4.2 (Golden Helix Inc., Bozeman, MT, USA). Association tests for allele and genotype frequencies for each SNP were performed by chi-squared test, using Helix Tree software v.6.4.2. OR were calculated with 95% CI. Statistical significance was set at p < 0.05.

Assuming a population prevalence of 0.2% and minor allele frequencies of 0.35–0.45, the study had 80% power to detect an additive association with OR of 1.35, a dominant association with OR of 1.6, and a recessive association with OR of 1.65, at the 5% significance level (Quanto software v.1.2.4, University of Southern California, Los Angeles, CA, USA).

RESULTS

From the 456 cases and 300 controls, 387 (85%) and 20 (7%), respectively, were found to be HLA-B27-positive.

Both SNP, rs10865331 and rs2242944, were in HWE or close to HWE in controls (p = 0.38 and 0.88, respectively) and cases (p = 0.02 and 0.83, respectively). The genotyping call rate was 0.98, with 449 and 295 genotypes obtained for rs10865331 and 454 and 290 for rs2242944, in cases and controls, respectively.

Results of allele and genotype comparisons are given in Table 2. A significant association was found for rs10865331 at both allele (p = 0.039) and genotype (p = 0.016) levels (Table 2). Specifically, the A allele was found to confer susceptibility to AS with an OR of 1.25 (95% CI 1.01–1.54). We observed a significant genotype association under the recessive model (OR 1.74, 95% CI 1.18–2.54; p = 0.004), but not under the dominant model (OR 1.10, 95% CI 0.80–1.51; p = 0.53). Neither the allele (p = 0.105) nor the genotype (p = 0.274) frequencies of rs2242944 were significantly different between patients and controls. In this case, we did not find significant associations under either the dominant model (OR 1.24, 95% CI 0.92–1.68; p = 0.16) or the recessive model (OR 1.3, 95% CI 0.83–2.02; p = 0.24).

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Table 2.

Genotype and allele association analysis of SNP rs10865331 and rs2242944 in patients with AS and spondyloarthritis-free controls. Study population consisted of 456 white Spanish patients with AS who fulfilled the modified New York criteria and 300 spondyloarthritis-free Spanish controls. The 2 SNP, rs10865331 and rs2242944, were genotyped by competitive allele-specific PCR.

We did not perform an association test based on carriage of HLA-B27, since this analysis was highly limited by the small number of B27-positive controls (n = 20) and B27-negative cases (n = 69).

DISCUSSION

We examined in a Spanish population the association with AS of 2 SNP recently identified in a GWAS, rs10865331 (2p15) and rs2242944 (21q22). We replicated for the first time in an independent cohort the association of rs10865331 with AS. No association was seen with rs2242944. This lack of association could be due to a sample size limitation, given the modest statistical power of our study, or to the different ethnic origins of the population, as seen for other SNP associated with AS13.

Both SNP, rs2242944 (2p15) and rs10865331 (21q22), map to intergenic regions where no known protein-coding gene has been described. Several recent genetic studies have shown the involvement of intergenic genomic regions and SNP in autoimmune diseases14,15. According to HapMap CEU genotype data (HapMap #27, on NCBI B36 assembly), rs10865331 and rs2242944 are not contained within the linkage disequilibrium (LD) blocks of their neighboring genes. Thus, it appears unlikely that these SNP are associated with AS due to LD with a causal variant in a neighboring gene.

The closest protein-coding genes to rs10865331 are B3GNT2 (UDP-GlcNAc: betaGal beta-1,3-N-acetylglucosaminyltransferase 1) and COMMD1 (copper metabolism domain containing 1), which codify for a type II transmembrane protein involved in the biosynthesis of poly-N-acetyllactosamine chains and for a protein known to inhibit nuclear factor-κB activation, respectively. The nearest gene to rs2242944 is PSMG1 (proteasome assembly chaperone 1).

We tested whether rs10865331 and rs2242944 were located in putative transcription factor binding sites (TFBS) using the MAPPER platform (http://mapper.chip.org) and whether the SNP could disrupt the TFBS. Regarding rs10865331, a putative TFBS for the transcription factor Tal1beta-E47S (score 6 and e-value 1.4) is predicted only if the G allele is present. If the susceptibility A allele is present, the binding of Tal1beta-E47S to this region is predicted to be disrupted. Since this putative TFBS is located downstream of COMMD1 and B3GNT2 genes, we hypothesize that it could be part of a long-range transcriptional regulatory element involved in the regulation of these neighboring genes or, alternatively, it could act as a proximal promoter element for a not yet identified protein coding gene in this region. We did not find any disruption of predicted TFBS for rs2242944, so we hypothesize that this SNP could be involved in AS pathogenesis-modifying gene expression through effects on noncoding RNA.

Our study provides evidence of the association of the rs10865331 intergenic variant with AS in a Spanish population, replicating the results recently obtained in a GWAS. Further research is necessary to unravel the functional significance and the biological processes altered by this intergenic AS-associated SNP.

Footnotes

  • Supported by the Ministerio de Ciencia e Innovación: Proyect PSE-01000-2006-1.

  • Accepted for publication July 6, 2010.

REFERENCES

  1. 1.↵
    1. Caffrey MF,
    2. James DC
    . Human lymphocyte antigen association in ankylosing spondylitis. Nature 1973;242:121.
    OpenUrlCrossRefPubMed
  2. 2.↵
    1. Burton PR,
    2. Clayton DG,
    3. Cardon LR,
    4. Craddock N,
    5. Deloukas P,
    6. Duncanson A,
    7. et al.
    Association scan of 14,500 nonsynonymous SNPs in four diseases identifies autoimmunity variants. Nat Genet 2007;39:1329–37.
    OpenUrlCrossRefPubMed
  3. 3.↵
    1. Pazar B,
    2. Safrany E,
    3. Gergely P,
    4. Szanto S,
    5. Szekanecz Z,
    6. Poor G
    . Association of ARTS1 gene polymorphisms with ankylosing spondylitis in the Hungarian population: the rs27044 variant is associated with HLA-B*2705 subtype in Hungarian patients with ankylosing spondylitis. J Rheumatol 2010;37:379–84.
    OpenUrlAbstract/FREE Full Text
  4. 4.↵
    1. Brown MA,
    2. Edwards S,
    3. Hoyle E,
    4. Campbell S,
    5. Laval S,
    6. Daly AK,
    7. et al.
    Polymorphisms of the CYP2D6 gene increase susceptibility to ankylosing spondylitis. Hum Mol Genet 2000;9:1563–6.
    OpenUrlAbstract/FREE Full Text
  5. 5.↵
    1. Timms AE,
    2. Crane AM,
    3. Sims AM,
    4. Cordell HJ,
    5. Bradbury LA,
    6. Abbott A,
    7. et al.
    The interleukin 1 gene cluster contains a major susceptibility locus for ankylosing spondylitis. Am J Hum Genet 2004;75:587–95.
    OpenUrlCrossRefPubMed
  6. 6.↵
    1. Safrany E,
    2. Pazar B,
    3. Csongei V,
    4. Jaromi L,
    5. Polgar N,
    6. Sipeky C,
    7. et al.
    Variants of the IL23R gene are associated with ankylosing spondylitis but not with Sjögren syndrome in Hungarian population samples. Scand J Immunol 2009;70:68–74.
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Reveille JD,
    2. Sims AM,
    3. Maksymowych W,
    4. Ward M,
    5. Stone M,
    6. Rahman P,
    7. et al.
    Genomewide association study in ankylosing spondylitis identifies major non-MHC genetic determinants of disease susceptibility [abstract]. Arthritis Rheum 2008;58 Suppl:S609.
    OpenUrl
  8. 8.↵
    1. Reveille JD,
    2. Sims AM,
    3. Danoy P,
    4. Evans DM,
    5. Leo P,
    6. Pointon JJ,
    7. et al.
    Genome-wide association study of ankylosing spondylitis identifies non-MHC susceptibility loci. Nat Genet 2010;42:123–7.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Collantes E,
    2. Zarco P,
    3. Muñoz E,
    4. Juanola X,
    5. Mulero J,
    6. Fernández-Sueiro JL,
    7. et al.
    Disease pattern of spondyloarthropathies in Spain: description of the first national registry (REGISPONSER) extended report. Rheumatology 2007;46:1309–15.
    OpenUrlAbstract/FREE Full Text
  10. 10.↵
    1. van der Linden S,
    2. Valkenburg HA,
    3. Cats A
    . Evaluation of diagnostic criteria for ankylosing spondylitis. A proposal for modification of the New York criteria. Arthritis Rheum 1984;27:361–8.
    OpenUrlPubMed
  11. 11.↵
    1. Olerup O
    . HLA-B27 typing by a group-specific PCR amplification. Tissue Antigens 1994;43:253–6.
    OpenUrlPubMed
  12. 12.↵
    1. Cho SY,
    2. Lee KG,
    3. Park SY,
    4. Lee HJ
    . Utility of in-house PCR for HLA-B27 typing: comparison of concordance rate between PCR kit and in-house PCR. Korean J Lab Med 2008;28:239–43.
    OpenUrlCrossRefPubMed
  13. 13.↵
    1. Pimentel-Santos FM,
    2. Ligeiro D,
    3. Matos M,
    4. Mourao AF,
    5. Sousa E,
    6. Pinto P,
    7. et al.
    Association of IL23R and ERAP1 genes with ankylosing spondylitis in a Portuguese population. Clin Exp Rheumatol 2009;27:800–6.
    OpenUrlPubMed
  14. 14.↵
    1. Rioux JD,
    2. Xavier RJ,
    3. Taylor KD,
    4. Silverberg MS,
    5. Goyette P,
    6. Huett A,
    7. et al.
    Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. Nat Genet 2007;39:596–604.
    OpenUrlCrossRefPubMed
  15. 15.↵
    1. Diéguez-González R,
    2. Calaza M,
    3. Pérez-Pampín E,
    4. Balsa A,
    5. Blanco FJ,
    6. Cañete JD,
    7. et al.
    Analysis of TNFAIP3, a feedback inhibitor of nuclear factor-kappa B and the neighbor intergenic 6q23 region in rheumatoid arthritis susceptibility. Arthritis Res Ther 2009;11:R42.
    OpenUrlCrossRefPubMed
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Association of the Intergenic Single-Nucleotide Polymorphism rs10865331 (2p15) with Ankylosing Spondylitis in a Spanish Population
ALEJANDRA SÁNCHEZ, MAGDALENA SZCZYPIORSKA, XAVIER JUANOLA, NEREA BARTOLOMÉ, JORDI GRATACÓS, PEDRO ZARCO, EDUARDO COLLANTES, MARTA ARTIEDA, ANTONIO MARTÍNEZ, DIEGO TEJEDOR, JUAN MULERO
The Journal of Rheumatology Nov 2010, 37 (11) 2345-2347; DOI: 10.3899/jrheum.100211

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Association of the Intergenic Single-Nucleotide Polymorphism rs10865331 (2p15) with Ankylosing Spondylitis in a Spanish Population
ALEJANDRA SÁNCHEZ, MAGDALENA SZCZYPIORSKA, XAVIER JUANOLA, NEREA BARTOLOMÉ, JORDI GRATACÓS, PEDRO ZARCO, EDUARDO COLLANTES, MARTA ARTIEDA, ANTONIO MARTÍNEZ, DIEGO TEJEDOR, JUAN MULERO
The Journal of Rheumatology Nov 2010, 37 (11) 2345-2347; DOI: 10.3899/jrheum.100211
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