Skip to main content

Main menu

  • Home
  • Content
    • First Release
    • Current
    • Archives
    • Collections
    • Audiovisual Rheum
    • COVID-19 and Rheumatology
  • Resources
    • Guide for Authors
    • Submit Manuscript
    • Author Payment
    • Reviewers
    • Advertisers
    • Classified Ads
    • Reprints and Translations
    • Permissions
    • Meetings
    • FAQ
    • Policies
  • Subscribers
    • Subscription Information
    • Purchase Subscription
    • Your Account
    • Terms and Conditions
  • About Us
    • About Us
    • Editorial Board
    • Letter from the Editor
    • Duncan A. Gordon Award
    • GDPR Policy
    • Accessibility
  • Contact Us
  • JRheum Supplements
  • Services

User menu

  • My Cart
  • Log In

Search

  • Advanced search
The Journal of Rheumatology
  • JRheum Supplements
  • Services
  • My Cart
  • Log In
The Journal of Rheumatology

Advanced Search

  • Home
  • Content
    • First Release
    • Current
    • Archives
    • Collections
    • Audiovisual Rheum
    • COVID-19 and Rheumatology
  • Resources
    • Guide for Authors
    • Submit Manuscript
    • Author Payment
    • Reviewers
    • Advertisers
    • Classified Ads
    • Reprints and Translations
    • Permissions
    • Meetings
    • FAQ
    • Policies
  • Subscribers
    • Subscription Information
    • Purchase Subscription
    • Your Account
    • Terms and Conditions
  • About Us
    • About Us
    • Editorial Board
    • Letter from the Editor
    • Duncan A. Gordon Award
    • GDPR Policy
    • Accessibility
  • Contact Us
  • Follow jrheum on Twitter
  • Visit jrheum on Facebook
  • Follow jrheum on LinkedIn
  • Follow jrheum on RSS
Research ArticleArticle

Vascular Endothelial Growth Factor in Rabbits During Development of Corticosteroid-Induced Osteonecrosis: A Controlled Experiment

TAMON KABATA, TADAMI MATSUMOTO, SHINICHI YAGISHITA, TOMOHIKO WAKAYAMA, SHOICHI ISEKI and KATSURO TOMITA
The Journal of Rheumatology December 2008, 35 (12) 2383-2390; DOI: https://doi.org/10.3899/jrheum.070838
TAMON KABATA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: tamonkabata@aol.com
TADAMI MATSUMOTO
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
SHINICHI YAGISHITA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
TOMOHIKO WAKAYAMA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
SHOICHI ISEKI
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
KATSURO TOMITA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • References
  • Info & Metrics
  • PDF
  • eLetters
PreviousNext
Loading

Abstract

Objective

Vascular endothelial growth factor (VEGF) is an angiogenic promoter that is rapidly induced as a response to local hypoxia. We investigated VEGF expression in rabbits in a controlled experiment to clarify the onset of ischemic events in corticosteroid-induced osteonecrosis (ON).

Methods

Ninety-nine mature Japanese white rabbits were divided into 6 treatment groups and an untreated control group. The treatment groups received a single intramuscular injection of 4 mg/kg methylprednisolone acetate; they were euthanized at different times, and tissue samples were obtained from their femora. We examined the development of ON and the expression of VEGF using histopathology, immunohistochemistry, Northern blot analysis, and Western blot analysis.

Results

On histopathological examination, the earliest indication of ON was 5 days after the corticosteroid treatment. The frequency of ON occurrence reached a plateau at or after Week 1. VEGF expression was accompanied by the development of ON. VEGF-positive cells detected by immunohistochemistry were found among bone marrow cells, frequently located in the area surrounding ON, suggesting that VEGF production was switched on as a result of the ischemic events that cause ON. The level of VEGF-mRNA expression indicated by Northern blot analysis peaked at 3 days after the corticosteroid treatment and decreased gradually to the levels present in the control group at 7 days after treatment. Western blot analysis revealed VEGF protein production at 3 days after the corticosteroid treatments. Levels of VEGF expression 2 weeks or more after the corticosteroid treatment were almost the same as in the control group.

Conclusion

We observed early expression of VEGF in the cells around the corticosteroid-induced ON lesions in rabbits. These results suggest that the ischemic events that cause ON begin soon after the initial corticosteroid treatment.

Key Indexing Terms:
  • OSTEONECROSIS
  • CORTICOSTEROIDS
  • RABBITS
  • ISCHEMIC EVENT
  • VASCULAR ENDOTHELIAL GROWTH FACTOR
  • DISEASE ONSET

Footnotes

  • T. Kabata, MD, PhD, Assistant Professor, Department of Orthopaedic Surgery, Kanazawa University School of Medicine; T. Matsumoto, MD, PhD, Professor, Department of Orthopaedic Surgery, Kanazawa Medical University; S. Yagishita, MD, PhD, Department of Orthopaedic Surgery, Kanazawa University School of Medicine; T. Wakayama, MD, PhD, Associate Professor; S. Iseki, MD, PhD, Associate Professor, Department of Anatomy (I), Kanazawa University School of Medicine; K. Tomita, MD, PhD, Professor, Department of Orthopaedic Surgery, Kanazawa University School of Medicine.

  • Supported by the Japanese Investigation Committee for Osteonecrosis of Femoral Head, under the auspices of the Ministry of Health and Welfare of Japan.

    • Accepted for publication July 10, 2008.
PreviousNext
Back to top

In this issue

The Journal of Rheumatology
Vol. 35, Issue 12
1 Dec 2008
  • Table of Contents
  • Table of Contents (PDF)
  • Index by Author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Rheumatology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Vascular Endothelial Growth Factor in Rabbits During Development of Corticosteroid-Induced Osteonecrosis: A Controlled Experiment
(Your Name) has forwarded a page to you from The Journal of Rheumatology
(Your Name) thought you would like to see this page from the The Journal of Rheumatology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Vascular Endothelial Growth Factor in Rabbits During Development of Corticosteroid-Induced Osteonecrosis: A Controlled Experiment
TAMON KABATA, TADAMI MATSUMOTO, SHINICHI YAGISHITA, TOMOHIKO WAKAYAMA, SHOICHI ISEKI, KATSURO TOMITA
The Journal of Rheumatology Dec 2008, 35 (12) 2383-2390; DOI: 10.3899/jrheum.070838

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

 Request Permissions

Share
Vascular Endothelial Growth Factor in Rabbits During Development of Corticosteroid-Induced Osteonecrosis: A Controlled Experiment
TAMON KABATA, TADAMI MATSUMOTO, SHINICHI YAGISHITA, TOMOHIKO WAKAYAMA, SHOICHI ISEKI, KATSURO TOMITA
The Journal of Rheumatology Dec 2008, 35 (12) 2383-2390; DOI: 10.3899/jrheum.070838
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Save to my folders

Jump to section

  • Article
  • References
  • Info & Metrics
  • PDF
  • eLetters

Related Articles

Cited By...

More in this TOC Section

  • Hospitalization Rates Are Highest in the First 5 Years of Systemic Sclerosis: Results From a Population-based Cohort (1980–2016)
  • Relationship Between Depression and Disease Activity in United States Veterans With Early Rheumatoid Arthritis Receiving Methotrexate
  • Hospitalized Infections in People With Osteoarthritis: A National US Study
Show more Articles

Similar Articles

Content

  • First Release
  • Current
  • Archives
  • Collections
  • Audiovisual Rheum
  • COVID-19 and Rheumatology

Resources

  • Guide for Authors
  • Submit Manuscript
  • Author Payment
  • Reviewers
  • Advertisers
  • Classified Ads
  • Reprints and Translations
  • Permissions
  • Meetings
  • FAQ
  • Policies

Subscribers

  • Subscription Information
  • Purchase Subscription
  • Your Account
  • Terms and Conditions

More

  • About Us
  • Contact Us
  • My Alerts
  • My Folders
  • RSS Feeds
The Journal of Rheumatology
The content of this site is intended for health care professionals.
Copyright © 2016 by The Journal of Rheumatology Publishing Co. Ltd.
Print ISSN: 0315-162X; Online ISSN: 1499-2752
Powered by HighWire