Skip to main content

Main menu

  • Home
  • Content
    • First Release
    • Current
    • Archives
    • Collections
    • Audiovisual Rheum
    • COVID-19 and Rheumatology
  • Resources
    • Guide for Authors
    • Submit Manuscript
    • Author Payment
    • Reviewers
    • Advertisers
    • Classified Ads
    • Reprints and Translations
    • Permissions
    • Meetings
    • FAQ
    • Policies
  • Subscribers
    • Subscription Information
    • Purchase Subscription
    • Your Account
    • Terms and Conditions
  • About Us
    • About Us
    • Editorial Board
    • Letter from the Editor
    • Duncan A. Gordon Award
    • GDPR Policy
    • Accessibility
  • Contact Us
  • JRheum Supplements
  • Services

User menu

  • My Cart
  • Log In

Search

  • Advanced search
The Journal of Rheumatology
  • JRheum Supplements
  • Services
  • My Cart
  • Log In
The Journal of Rheumatology

Advanced Search

  • Home
  • Content
    • First Release
    • Current
    • Archives
    • Collections
    • Audiovisual Rheum
    • COVID-19 and Rheumatology
  • Resources
    • Guide for Authors
    • Submit Manuscript
    • Author Payment
    • Reviewers
    • Advertisers
    • Classified Ads
    • Reprints and Translations
    • Permissions
    • Meetings
    • FAQ
    • Policies
  • Subscribers
    • Subscription Information
    • Purchase Subscription
    • Your Account
    • Terms and Conditions
  • About Us
    • About Us
    • Editorial Board
    • Letter from the Editor
    • Duncan A. Gordon Award
    • GDPR Policy
    • Accessibility
  • Contact Us
  • Follow jrheum on Twitter
  • Visit jrheum on Facebook
  • Follow jrheum on LinkedIn
  • Follow jrheum on RSS
Research ArticleArticle

A Combination of Granulocyte Colony-Stimulating Factor and Stem Cell Factor Ameliorates Steroid- Associated Osteonecrosis in Rabbits

XINGHUO WU, SHUHUA YANG, DEYU DUAN, XIANZHE LIU, YUKUN ZHANG, JING WANG, CAO YANG and SONG JIANG
The Journal of Rheumatology November 2008, 35 (11) 2241-2248; DOI: https://doi.org/10.3899/jrheum.071209
XINGHUO WU
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
SHUHUA YANG
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DEYU DUAN
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
XIANZHE LIU
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YUKUN ZHANG
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
JING WANG
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
CAO YANG
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: shuhua196@yahoo.com.cn
SONG JIANG
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • References
  • Info & Metrics
  • PDF
  • eLetters
PreviousNext
Loading

Abstract

Objective

Bone marrow-derived stem cells (BMSC) have been highlighted for the treatment of osteonecrosis (ON) before collapse of the femoral head. In our study, the potential of granulocyte colony-stimulating factor/stem cell factor (G-CSF/SCF)-mobilized BMSC to repair steroid-associated ON was assessed in rabbits.

Methods

ON was induced by low-dose lipopolysaccharide and subsequent pulsed high-dose methylprednisolone. Rabbits in the treated group were subjected to subcutaneous injections of GCSF at a dose of 100 μg/kg and SCF 25 μg/kg per day for 5 days; rabbits in the control group were given saline. Blood samples were collected and serum osteocalcin was detected by ELISA. Radiological analysis was performed by magnetic resonance imaging (MRI). Then bilateral femora and humeri were harvested and processed to paraffin sections and hard-tissue sections for immunohistochemical, histologic, and histomorphometric analysis.

Results

The mean number of leukocytes and relative numbers of mononuclear cells increased significantly after mobilization. All rabbits displayed a marked increase in osteocalcin protein expression in response to G-CSF/SCF. MRI scans showed a reactive interface between the necrotic and reparative zones after G-CSF/SCF administration. Quantitative analysis showed that new vessel formation was 3.3-fold greater and vessel density was 2.6-fold greater in the treatment group than the control group. The histologic and histomorphometric analysis revealed that the new bone volume was significantly higher in the G-SCF/SCF group than in the control group at 4 weeks.

Conclusion

G-CSF/SCF-induced mobilization of BMSC in the necrotic foci may represent a promising strategy for promoting functional bone repair of early-stage ON.

Key Indexing Terms:
  • BONE MARROW-DERIVED STEM CELL
  • STEM CELL FACTOR
  • OSTEONECROSIS
  • GRANULOCYTE COLONY-STIMULATING FACTOR
  • MOBILIZATION

Footnotes

  • X. Wu, MD; S. Yang, MD, Professor, PhD Advisor; D. Duan, MD; X. Liu, MM; Y. Zhang, MD; J. Wang, MD; C. Yang, MD; S. Jiang, MM, Department of Orthopaedics, Union Hospital, Tongji Medical College.

    • Accepted for publication March 28, 2008.
PreviousNext
Back to top

In this issue

The Journal of Rheumatology
Vol. 35, Issue 11
1 Nov 2008
  • Table of Contents
  • Index by Author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word about The Journal of Rheumatology.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
A Combination of Granulocyte Colony-Stimulating Factor and Stem Cell Factor Ameliorates Steroid- Associated Osteonecrosis in Rabbits
(Your Name) has forwarded a page to you from The Journal of Rheumatology
(Your Name) thought you would like to see this page from the The Journal of Rheumatology web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
A Combination of Granulocyte Colony-Stimulating Factor and Stem Cell Factor Ameliorates Steroid- Associated Osteonecrosis in Rabbits
XINGHUO WU, SHUHUA YANG, DEYU DUAN, XIANZHE LIU, YUKUN ZHANG, JING WANG, CAO YANG, SONG JIANG
The Journal of Rheumatology Nov 2008, 35 (11) 2241-2248; DOI: 10.3899/jrheum.071209

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

 Request Permissions

Share
A Combination of Granulocyte Colony-Stimulating Factor and Stem Cell Factor Ameliorates Steroid- Associated Osteonecrosis in Rabbits
XINGHUO WU, SHUHUA YANG, DEYU DUAN, XIANZHE LIU, YUKUN ZHANG, JING WANG, CAO YANG, SONG JIANG
The Journal of Rheumatology Nov 2008, 35 (11) 2241-2248; DOI: 10.3899/jrheum.071209
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One
Save to my folders

Jump to section

  • Article
  • References
  • Info & Metrics
  • PDF
  • eLetters

Related Articles

Cited By...

More in this TOC Section

  • Salivary Gland Focus Score Is Associated With Myocardial Fibrosis in Primary Sjögren Syndrome Assessed by a Cardiac Magnetic Resonance Approach
  • National Priorities for High-quality Rheumatology Transition Care for Youth in Canada
  • The Effect of Creatine Supplementation on Muscle Function in Childhood Myositis: A Randomized, Double-blind, Placebo-controlled Feasibility Study
Show more Article

Similar Articles

Content

  • First Release
  • Current
  • Archives
  • Collections
  • Audiovisual Rheum
  • COVID-19 and Rheumatology

Resources

  • Guide for Authors
  • Submit Manuscript
  • Author Payment
  • Reviewers
  • Advertisers
  • Classified Ads
  • Reprints and Translations
  • Permissions
  • Meetings
  • FAQ
  • Policies

Subscribers

  • Subscription Information
  • Purchase Subscription
  • Your Account
  • Terms and Conditions

More

  • About Us
  • Contact Us
  • My Alerts
  • My Folders
  • RSS Feeds
The Journal of Rheumatology
The content of this site is intended for health care professionals.
Copyright © 2016 by The Journal of Rheumatology Publishing Co. Ltd.
Print ISSN: 0315-162X; Online ISSN: 1499-2752
Powered by HighWire