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Abstract

High diagnostic value of anticalpastatin autoantibodies in rheumatoid arthritis detected by ELISA using human erythrocyte calpastatin as antigen.

Sachiko Iwaki-Egawa, Hiroaki Matsuno, Kazuo Yudoh, Fujio Nakazawa, Kyousuke Miyazaki, Akira Ochiai, Shunsei Hirohata, Masatoshi Shimizu and Yasuhiro Watanabe
The Journal of Rheumatology January 2004, 31 (1) 17-22;
Sachiko Iwaki-Egawa
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Hiroaki Matsuno
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Kazuo Yudoh
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Fujio Nakazawa
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Kyousuke Miyazaki
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Akira Ochiai
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Shunsei Hirohata
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Masatoshi Shimizu
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Yasuhiro Watanabe
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Abstract

OBJECTIVE: To develop a quantitative method of measuring autoantibodies against human calpastatin in rheumatoid arthritis (RA) and to determine their diagnostic value compared with other autoimmune and articular diseases. METHODS: We performed a highly sensitive ELISA for IgG and IgM anticalpastatin autoantibodies in human sera using human erythrocyte calpastatin as an antigen. Samples were diluted 1:2000 for the measurement of IgG and 1:400 for IgM. RESULTS: IgG anticalpastatin antibodies were found in the sera of 48 of 58 patients (82.8%) with RA. In contrast, IgG anticalpastatin antibodies were found in the sera of only 2 of 11 (8.3%) patients with osteoarthritis (OA). Compared to sera from patients with other autoimmune diseases, anticalpastatin antibody sensitivity for RA was better than that of systemic lupus erythematosus (5.6%), systemic sclerosis (0%), mixed connective tissue disease (0%), and Sjögren's syndrome (20%). IgG anticalpastatin antibodies also showed high specificity (96.1%) for RA. Almost 90% of patients with RA were positive for IgG or IgM anticalpastatin antibodies. CONCLUSION: We have developed a simple, sensitive, specific, and quantitative ELISA for anticalpastatin antibodies that may have a high diagnostic value for RA.

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The Journal of Rheumatology
Vol. 31, Issue 1
1 Jan 2004
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High diagnostic value of anticalpastatin autoantibodies in rheumatoid arthritis detected by ELISA using human erythrocyte calpastatin as antigen.
Sachiko Iwaki-Egawa, Hiroaki Matsuno, Kazuo Yudoh, Fujio Nakazawa, Kyousuke Miyazaki, Akira Ochiai, Shunsei Hirohata, Masatoshi Shimizu, Yasuhiro Watanabe
The Journal of Rheumatology Jan 2004, 31 (1) 17-22;

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High diagnostic value of anticalpastatin autoantibodies in rheumatoid arthritis detected by ELISA using human erythrocyte calpastatin as antigen.
Sachiko Iwaki-Egawa, Hiroaki Matsuno, Kazuo Yudoh, Fujio Nakazawa, Kyousuke Miyazaki, Akira Ochiai, Shunsei Hirohata, Masatoshi Shimizu, Yasuhiro Watanabe
The Journal of Rheumatology Jan 2004, 31 (1) 17-22;
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