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Abstract

Gene marking in adeno-associated virus vector infected periosteum derived cells for cartilage repair.

Naomi Kobayashi, Tomihisa Koshino, Masaaki Uesugi, Naoki Yokoo, Ke-Qin Xin, Kenji Okuda, Hiroaki Mizukami, Keiya Ozawa and Tomoyuki Saito
The Journal of Rheumatology October 2002, 29 (10) 2176-2180;
Naomi Kobayashi
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Tomihisa Koshino
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Masaaki Uesugi
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Naoki Yokoo
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Ke-Qin Xin
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Kenji Okuda
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Hiroaki Mizukami
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Keiya Ozawa
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Tomoyuki Saito
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Abstract

OBJECTIVE: To evaluate both the potential for transferring genes to periosteal cells using an adeno-associated virus (AAV) vector and the potential for gene expression after transplantation of those cells to a cartilage defect in vivo. METHODS: Periosteum was obtained from the tibia of 6-week-old rabbits and enzymatically digested. The isolated periosteum derived cells were cultured and the subconfluence cells were infected with a recombinant AAV expressing the LacZ gene (AAV-LacZ). Collagen gel containing the LacZ transferred, periosteum derived cells was transplanted into a full thickness articular cartilage defect in 10 rabbits. RESULTS: Infected cells still growing on the plate continued to express LacZ at least 12 weeks after AAV infection, with the highest percentage of LacZ positive cells reaching 74.4%. The LacZ positive cells were recognized at the transplant sites in 8 out of 10 knees. CONCLUSION: Gene expression in periosteum derived cells was sustained in vitro for at least 12 weeks using the AAV vector, and for 2 weeks ex vivo after transplantation into a cartilage defect.

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The Journal of Rheumatology
Vol. 29, Issue 10
1 Oct 2002
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Gene marking in adeno-associated virus vector infected periosteum derived cells for cartilage repair.
Naomi Kobayashi, Tomihisa Koshino, Masaaki Uesugi, Naoki Yokoo, Ke-Qin Xin, Kenji Okuda, Hiroaki Mizukami, Keiya Ozawa, Tomoyuki Saito
The Journal of Rheumatology Oct 2002, 29 (10) 2176-2180;

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Gene marking in adeno-associated virus vector infected periosteum derived cells for cartilage repair.
Naomi Kobayashi, Tomihisa Koshino, Masaaki Uesugi, Naoki Yokoo, Ke-Qin Xin, Kenji Okuda, Hiroaki Mizukami, Keiya Ozawa, Tomoyuki Saito
The Journal of Rheumatology Oct 2002, 29 (10) 2176-2180;
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