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(receptor integrin subunit )Tj
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[(rheumatoid arthritis. )54.8 (Ann Rheum Dis 1998;57:559-65.)]TJ
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[(30.)-515.6 (Sonnenber)17.7 (g )54.8 (A, Linders C, Daams JH, Kennel SJ. )17.7 (The )]TJ
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[(31.)-515.6 (Prieto J, Eklund )54.8 (A, Patarroyo M. Regulated expression of integrins and)]TJ
1.7655 -1.25 Td
[(other adhesion molecules during dif)17.7 (ferentiation of monocytes into)]TJ
T*
[(macrophages. Cell Immunol 1994;156:191-21)36.8 (1.)]TJ
-1.7655 -1.25 Td
[(32.)-515.6 (Mulherin D, Fitzgerald O, Bresnihan B. Synovial tissue macrophage)]TJ
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[(populations and articular damage in rheumatoid arthritis. )54.8 (Arthritis)]TJ
0 Tc T*
[(Rheum 1996;39:1)36.9 (15-24.)]TJ
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[(33.)-515.6 (Hendler PL, Lavoie PE, )17.7 (W)79.9 (erb Z, Chan J, Seaman )17.7 (WE. Human)]TJ
1.7655 -1.25 Td
(synovial dendritic cells. Direct observation of transition to fibroblast\
s.)Tj
T*
(J Rheumatol 1985;12:660-4.)Tj
-1.7655 -1.25 Td
[(34.)-515.6 (Dayer JM, Krane SM, Russell RG, Robinson DR. Production of)]TJ
1.7655 -1.25 Td
(collagenase and prostaglandins by isolated adherent rheumatoid)Tj
T*
[(synovial cells. Proc Natl )54.8 (Acad Sci USA)-220.2 (1976;73:945-9.)]TJ
-1.7655 -1.25 Td
[(35.)-515.6 (W)79.9 (oolley DE, Harris ED Jr)39.7 (, Mainardi CL, Brinckerhof)17.7 (f CE.)]TJ
1.7655 -1.25 Td
(Collagenase immunolocalization in cultures of rheumatoid synovial)Tj
T*
(cells. Science 1978;200:773-5.)Tj
-1.7655 -1.25 Td
[(36.)-515.6 (Sack U, Kuhn H, Ermann J, et al. Synovial tissue implants from)]TJ
1.7655 -1.25 Td
(patients with rheumatoid arthritis cause cartilage destruction in knee)Tj
T*
(joints of SCID.bg mice. J Rheumatol 1994;21:10-6.)Tj
-1.7655 -1.25 Td
[(37.)-515.6 (Zvaifler NJ, Firestein GS. Pannus and pannocytes. )54.8 (Alternative models)]TJ
1.7655 -1.25 Td
[(of joint destruction in rheumatoid arthritis. )54.8 (Arthritis Rheum)]TJ
0 Tc 0 Tw T*
(1994;37:783-9.)Tj
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(markers)Tj
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[(grin. )17.7 (When the synovial cells are cultured )]TJ
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18.165 0 Td
[(in vitr)36.8 (o)]TJ
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/T1_1 1 Tf
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(\002)Tj
/T1_0 1 Tf
-0.00011 Tc 0.114 Tw [0.1 (6 integrin expression in type B cells. It is)]TJ
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0.0334 Tw T*
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/T1_2 1 Tf
0.02499 Tw T*
(in vivo)Tj
/T1_0 1 Tf
0 Tc 0 Tw [-0.2 (.)]TJ
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0.0312 Tw T*
(It has been suggested that pannocytes are transformed type B)Tj
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(cells)Tj
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(26,37)Tj
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(rheumatoid disease, type B cells are not well characterized.)Tj
0.2119 Tw T*
[(Our results indicate that synovial type B cells dif)17.7 (fer from)]TJ
0.2605 Tw T*
(other fibroblastic cells since in both inflamed and nonin-)Tj
0.1752 Tw T*
(flamed synovium they contain )Tj
/T1_1 1 Tf
0 Tc 0 Tw 13.0867 0 Td
(\002)Tj
/T1_0 1 Tf
-0.00011 Tc 0.17529 Tw [0.1 (6 integrin. Synovial lining)]TJ
0.1985 Tw -13.0866 -1.2 Td
(cells are surrounded by laminin, the ligand for )Tj
/T1_1 1 Tf
0 Tc 0 Tw 20.3306 0 Td
(\002)Tj
/T1_0 1 Tf
-0.00011 Tc 0.1985 Tw [0.1 (6\2471 inte-)]TJ
0 Tw -20.3306 -1.2 Td
(grin)Tj
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(5,6)Tj
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(. Studies have shown the importance of integrin medi-)Tj
0.37331 Tw -2.4231 -1.2 Td
(ated cell adhesion for cell proliferation, phenotype, and)Tj
0.07539 Tw T*
(survival, emphasizing the importance of type B cell\320laminin)Tj
0.0119 Tc 0.36301 Tw T*
[(interaction. Further)39.8 (, the integrin\320ligand interaction may)]TJ
0.0146 Tc 0.36031 Tw T*
(trigger matrix metalloproteinase expression and initiate)Tj
-0.00011 Tc 0.1407 Tw T*
[(matrix destruction or remodeling. )17.8 (That type )54.8 (A)-335.8 (cells did not)]TJ
0 Tw T*
(express )Tj
/T1_1 1 Tf
0 Tc 3.298 0 Td
(\002)Tj
/T1_0 1 Tf
-0.00011 Tc 0.0497 Tw [0.1 (6\2471 integrin laminin receptor may contribute to the)]TJ
0.09261 Tw -3.298 -1.2 Td
(mobility of macrophages and to their ability to interact with)Tj
0.0163 Tw T*
[(other matrix components. )17.7 (The unique features of the synovial)]TJ
0.0694 Tw T*
(lining cell types must be taken into account when the patho-)Tj
0.02499 Tw T*
(logical mechanisms of rheumatoid disease are studied.)Tj
/T1_3 1 Tf
0 Tw 0 -2.4 TD
(ACKNOWLEDGMENT)Tj
/T1_0 1 Tf
0.0029 Tw 7.76 0 0 8 54 259.1616 Tm
[(W)79.9 (e thank Dr)54.8 (. )54.8 (Arnoud Sonnenber)17.7 (g for antibodies, and Dr)54.9 (. Matti Laato and Dr)54.8 (.)]TJ
0.05589 Tw 0 -1.25 TD
[(T)35 (uomas )36.7 (Yli-Jama for the patient samples. )17.7 (The expert technical assistance of)]TJ
0.10741 Tw T*
[(Marita Potila is gratefully acknowledged. Liisa Lempi\212inen and Dr)54.8 (. Hannu)]TJ
0.19299 Tw T*
[(Kalimo have given practical advice concerning the double reactions. )17.7 (The)]TJ
0.1293 Tw T*
[(entire staf)17.7 (f of the Laboratory of Electron Microscopy is acknowledged for)]TJ
0.02499 Tw T*
(technical help.)Tj
/T1_3 1 Tf
0 Tw 9.7 0 0 10 54 185.1616 Tm
(REFERENCES)Tj
/T1_0 1 Tf
0.02499 Tw 7.76 0 0 8 61.18 175.1616 Tm
[(1.)-515.6 (Edwards J, )17.7 (W)39.8 (illoughby DA. Demonstration of bone marrow derived)]TJ
1.2655 -1.25 Td
(cells in synovial lining by means of giant intracellular granulaes as)Tj
0 Tc T*
[(genetic markers. )54.8 (Ann Rheum Dis 1982;41:177-82.)]TJ
-0.00011 Tc -1.2655 -1.25 Td
[(2.)-515.6 (Edwards JCW)91.7 (, Sedgwick )54.8 (AD, )17.7 (W)39.9 (illoughby DA. Membrane properties)]TJ
1.2655 -1.25 Td
(and esterase activity of synovial lining cells, further evidence for a)Tj
T*
[(mononuclear phagocyte population. )54.8 (Ann Rheum Dis 1982;41:282-6.)]TJ
-1.2655 -1.25 Td
[(3.)-515.6 (Shiozawa S, Shiozawa K, Fujita )17.7 (T)74 (. Presence of HLA-DR antigens on)]TJ
1.2655 -1.25 Td
[(synovial type )54.8 (A)-220.1 (and B cells: an immunoelectron microscopic study in)]TJ
T*
(rheumatoid arthritis, osteoarthritis and normal traumatic joints.)Tj
T*
(Immunology 1983;50:587-94.)Tj
-1.2655 -1.25 Td
[(4.)-515.6 (M\237ller)19.7 (-Ladner U, Gay S, Gay RE. Cellular pathways of joint)]TJ
35.2861 79.921 Td
(destruction. Curr Opin Rheumatol 1995;9:213-20.)Tj
-1.2655 -1.25 Td
[(5.)-515.6 (Nikkari L, Haapasalmi K, )54.8 (Aho H, et al. Localization of the )]TJ
/T1_1 1 Tf
0 Tc 0 Tw 25.2815 0 Td
(\002)Tj
/T1_0 1 Tf
[0.1 (V)]TJ
-0.00011 Tc 0.02499 Tw -24.016 -1.25 Td
(subfamily of integrins and their putative ligands in synovial lining cel\
l)Tj
T*
[(layer)54.8 (. J Rheumatol 1995;22:16-23.)]TJ
-1.2655 -1.25 Td
[(6.)-515.6 (Pollock LE, Lalor P)110.7 (, Revell P)91.7 (A. )17.7 (T)69.9 (ype IV)-257.3 (collagen and laminin in the)]TJ
1.2655 -1.25 Td
[(synovial intimal layer: an immunohistochemical study)64.8 (. Rheumatol Int)]TJ
0 Tc 0 Tw T*
(1990;9:277-280.)Tj
-0.00011 Tc 0.02499 Tw -1.2655 -1.25 Td
[(7.)-515.6 (Cutolo M, Picasso M, Ponassi M, Sun MZ, Balza E. )17.7 (T)69.9 (enascin and)]TJ
1.2655 -1.25 Td
(fibronectin distribution in human normal and pathological synovium. )Tj
T*
(J Rheumatol 1992;19:1439-47.)Tj
-1.2655 -1.25 Td
[(8.)-515.6 (V)110.8 (artio )17.7 (T)74 (,)-0.1 ( )17.7 (V)110.8 (aheri )54.8 (A, von Essen R, Isom\212ki H, Stenman S. Fibronectin in)]TJ
1.2655 -1.25 Td
(synovial fluid and tissue in rheumatoid arthritis. Eur J Clin Invest)Tj
0 Tc 0 Tw T*
[(1981;1)36.9 (1:207-12.)]TJ
-0.00011 Tc 0.02499 Tw -1.2655 -1.25 Td
[(9.)-515.6 (Scott DL, Delamere JP)110.7 (, )17.7 (W)79.9 (alton KW)91.7 (. )17.7 (The distribution of fibronectin in)]TJ
1.2655 -1.25 Td
(the pannus in rheumatoid arthritis. Br J Exp Pathol 1981;62:362-8.)Tj
-1.7655 -1.25 Td
[(10.)-515.6 (Hynes RO. Integrins: versatility)64.8 (, modulation, and signaling in cell)]TJ
1.7655 -1.25 Td
[(adhesion. Cell 1992;69:1)36.8 (1-25.)]TJ
-1.7286 -1.25 Td
[(1)36.8 (1)-0.1 (.)-515.6 (Ruoslahti E. Integrins. J Clin Invest 1991;87:1-5.)]TJ
-0.0369 -1.25 Td
[(12.)-515.6 (Riikonen )17.7 (T)74 (,)-0.1 ( )17.7 (W)79.9 (estermarck J, Koivisto L, Brober)17.7 (g )54.8 (A, K\212h\212ri )17.7 (V)91.8 (-M,)]TJ
1.7655 -1.25 Td
(Heino J. )Tj
/T1_1 1 Tf
0 Tc 0 Tw [-0.5 (\002)]TJ
/T1_0 1 Tf
-0.00011 Tc 0.02499 Tw [0.1 (2\2471 integrin is a positive regulator of collagenase \(MMP-1\))]TJ
T*
(and collagen )Tj
/T1_1 1 Tf
0 Tc 0 Tw [-0.5 (\002)]TJ
/T1_0 1 Tf
-0.00011 Tc 0.02499 Tw [0.1 (1\(I\) gene expression. J Biol Chem 1995;270:13548-52.)]TJ
-1.7654 -1.25 Td
[(13.)-515.6 (Larjava H, L)54.8 (yons J, Salo )17.7 (T)74 (, et al. )54.8 (Anti-integrin antibodies induce type)]TJ
1.7655 -1.25 Td
[(IV)-257.3 (collagenase expression in keratinocytes. J Cell Physiol)]TJ
0 Tc 0 Tw T*
(1993;157:190-200.)Tj
-0.00011 Tc 0.02499 Tw -1.7655 -1.25 Td
[(14.)-515.6 (W)79.9 (erb Z, )17.7 (T)35 (remble PM, Behrendtsen O, Crowley E, Damsky CH. Signal)]TJ
1.7655 -1.25 Td
(transduction through the fibronectin receptor induces collagenase and)Tj
T*
(stromelysin gene expression. J Cell Biol 1989;109:877-89.)Tj
-1.7655 -1.25 Td
[(15.)-515.6 (Johnson BA, Haines GK, Harlow LA, Koch )54.8 (AE. )54.8 (Adhesion molecule)]TJ
1.7655 -1.25 Td
[(expression in human synovial tissue. )54.8 (Arthritis Rheum 1993;36:137-46.)]TJ
-1.7655 -1.25 Td
[(16.)-515.6 (Nikkari L, )54.8 (Aho H, )36.8 (Yli-Jama )17.7 (T)74 (, Larjava H, Jalkanen M, Heino J.)]TJ
1.7655 -1.25 Td
(Expression of integrin family of cell adhesion receptors in rheumatoid)Tj
0 Tc T*
[(synovium. )54.8 (Am J Pathol 1993;142:1019-27.)]TJ
-0.00011 Tc -1.7655 -1.25 Td
[(17.)-515.6 (Zvaifler NJ. Macrophages and the synovial lining. Scand J Rheumatol)]TJ
0 Tc 0 Tw 1.7655 -1.25 Td
(1995;101:67-75.)Tj
-0.00011 Tc 0.02499 Tw -1.7655 -1.25 Td
[(18.)-515.6 (Demaziere )54.8 (A, )54.8 (Athanasou NA. )54.8 (Adhesion receptors of intimal and)]TJ
1.7655 -1.25 Td
(subintimal cells of the normal synovial membrane. J Pathol 1992;)Tj
0 Tc 0 Tw T*
(168:209-15.)Tj
-0.00011 Tc 0.02499 Tw -1.7655 -1.25 Td
[(19.)-515.6 (Piril\212 L, Heino J. )54.8 (Altered integrin expression in rheumatoid synovial)]TJ
1.7655 -1.25 Td
(lining type B cell. In vitro regulation on )Tj
/T1_1 1 Tf
0 Tc 0 Tw 16.3907 0 Td
(\002)Tj
/T1_0 1 Tf
[0.1 (1\2471, )]TJ
/T1_1 1 Tf
2.6559 0 Td
(\002)Tj
/T1_0 1 Tf
0.02499 Tw [0.2 (6\2471 and )]TJ
/T1_1 1 Tf
0 Tw [0.1 (\002)]TJ
/T1_0 1 Tf
[0.1 (V\2475)]TJ
-0.00011 Tc 0.02499 Tw -19.0465 -1.25 Td
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-1.7655 -1.25 Td
[(20.)-515.6 (Arnett FC, Edworthy SM, Bloch DA, et al. )17.7 (The )54.8 (American Rheumatism)]TJ
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(Association 1987 revised criteria for the classification of rheumatoid)Tj
T*
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[(21.)-515.6 (Heino J, )17.7 (V)59.8 (iander M, Peltonen J, Kouri )17.7 (T)74 (.)-0.1 ( )54.8 (Adherent cells from)]TJ
1.7655 -1.25 Td
[(rheumatoid synovia: identity of HLA-DR positive stellate cells. )54.8 (Ann)]TJ
T*
[(Rheum Dis 1987;46:1)36.8 (14-20.)]TJ
-1.7655 -1.25 Td
[(22.)-515.6 (Sonnenber)17.7 (g )54.8 (A, Hogervorst F)79.7 (, Osterop )54.8 (A, )17.7 (V)110.8 (eltman FE. Identification)]TJ
1.7655 -1.25 Td
(and characterization of a novel antigen complex on mouse mammary)Tj
T*
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(J Biol Chem 1988;263:14030-8.)Tj
-0.00011 Tc -1.7655 -1.25 Td
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(integrins and basement membrane components by wound)Tj
T*
(keratinocytes. J Clin Invest 1993;92:1425-35.)Tj
-1.7655 -1.25 Td
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1.7655 -1.25 Td
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/T1_0 1 Tf
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[(of integrins in the development and sexual dif)17.7 (ferentiation of the mouse)]TJ
T*
[(testis. Dif)17.7 (ferentiation 1994;57:21-9.)]TJ
-1.7655 -1.25 Td
[(26.)-515.6 (Firestein GS. Invasive fibroblast-like synoviocytes in rheumatoid)]TJ
1.7655 -1.25 Td
[(arthritis. Passive responders or transformed aggressors. )54.8 (Arthritis)]TJ
0 Tc T*
(Rheum 1996;39:1781-90.)Tj
-0.00011 Tc -1.7655 -1.25 Td
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1.7655 -1.25 Td
[(Morphology and functional roles of synoviocytes in the joint. )54.8 (Arch)]TJ
T*
(Histol Cytol 2000;63:17-31.)Tj
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104.19 60.08 407.5 -10.83 re
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(other tissue)Tj
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(27,28)Tj
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(matrix metalloproteinases)Tj
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(12-14)Tj
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0.07919 Tw T*
(changes in integrin expression in rheumatoid synovial lining)Tj
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(cells)Tj
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(5,15-18,29)Tj
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(.)Tj
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(tive number of )Tj
/T1_2 1 Tf
0 Tc 0 Tw [-0.4 (\002)]TJ
/T1_1 1 Tf
-0.00011 Tc 0.12399 Tw [0.1 (6\2471 integrin positive cells is decreased)]TJ
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(15-17)Tj
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[(W)39.8 (ith light microscopy it is not possible to distinguish between)]TJ
-0.00169 Tw T*
[(type )54.9 (A)-193.3 (and type B cells by their morphology)64.8 (, and the localiza-)]TJ
0.03551 Tw T*
(tion of integrins in the 2 specific cell types has been undeter-)Tj
0.36549 Tw T*
(mined. Integrin )Tj
/T1_2 1 Tf
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/T1_1 1 Tf
-0.00011 Tc 0.3656 Tw [0.1 (6\2471 is typically a laminin receptor of)]TJ
0.26199 Tw T*
(endothelial cells, adipocytes, and macrophages and is not)Tj
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(expressed on fibroblasts)Tj
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(30,31)Tj
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[(. )17.7 (Thus the original hypothesis was)]TJ
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/T1_2 1 Tf
0 Tc 0 Tw [-0.3 (\002)]TJ
/T1_1 1 Tf
-0.00011 Tc 0.3125 Tw [0.1 (6 integrin. Our results,)]TJ
0.05769 Tw T*
[(however)39.7 (, unveiled the unique localization of adhesion recep-)]TJ
0.02499 Tw T*
(tors on synovial lining cells.)Tj
0.0289 Tw 1.2371 -1.2 Td
(The synovial lining seems to have at least 2 distinct popu-)Tj
0.11011 Tw -1.2371 -1.2 Td
(lations of macrophages)Tj
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(32)Tj
-0.00011 Tc 0.11011 Tw 9.7 0 0 10 152.3989 122.2556 Tm
(. Here, macrophage-type cells were)Tj
0.00011 Tw -10.1442 -1.2 Td
(studied more closely by using 2 macrophage markers. Human)Tj
0.0199 Tc 0.5097 Tw T*
[(alveolar macrophage marker)39.8 (, HAM56, can recognize)]TJ
-0.00011 Tc -0.0352 Tw T*
[(\322mature\323 macrophages. )54.9 (Another widely used marker of mono-)]TJ
0.36591 Tw T*
[(cytes and macrophages is CD68. )17.8 (W)79.9 (e show here that 2)]TJ
0.1749 Tw 27.2165 27.6907 Td
[(macrophage markers, HAM56 and CD68, are dif)17.7 (ferentially)]TJ
0.0444 Tw T*
[(located in inflamed synovium, whereas there is no dif)17.7 (ference)]TJ
0.0786 Tw T*
[(in their appearance in healthy synovium. )17.7 (The CD68 positive)]TJ
0.03081 Tw T*
(lining cells are more numerous than HAM56 positive cells in)Tj
0.03729 Tw T*
(inflamed synovium and they are often located on the superfi-)Tj
0.002 Tw T*
[(cial part of lining cell layer)54.9 (. )17.7 (The cellular functions of HAM56)]TJ
0.0047 Tc 0.37019 Tw T*
[(antigen and CD68 are unknown. Surprisingly)65.1 (, the cells)]TJ
-0.00011 Tc 0.0845 Tw T*
(expressing macrophage markers did not have )Tj
/T1_2 1 Tf
0 Tc 0 Tw 18.8323 0 Td
(\002)Tj
/T1_1 1 Tf
-0.00011 Tc 0.0845 Tw [0.1 (6 integrin on)]TJ
0.2361 Tw -18.8323 -1.2 Td
[(their surfaces. )17.7 (The )]TJ
/T1_2 1 Tf
0 Tc 0 Tw [0.1 (\002)]TJ
/T1_1 1 Tf
-0.00011 Tc 0.2361 Tw [0.1 (6 integrin positive cells were mainly)]TJ
-0.01579 Tw T*
(located in the basal areas of the lining cell layer and they were)Tj
0.09331 Tw T*
(negative for the macrophage markers. Immunolabeling elec-)Tj
0.23779 Tw T*
(tron microscopy showed that in rheumatoid synovium the)Tj
0.06219 Tw T*
[(cells considered as macrophages, or type )54.8 (A)-257.3 (cells, were nega-)]TJ
0.0787 Tw T*
(tive and the fibroblast-like type B cells were positive for )Tj
/T1_2 1 Tf
0 Tc 0 Tw 23.6114 0 Td
(\002)Tj
/T1_1 1 Tf
[0.1 (6)]TJ
-0.00011 Tc 0.073 Tw -23.6114 -1.2 Td
[(integrin. )17.7 (The results suggest that in RA)-268.2 (the disappearance of)]TJ
/T1_2 1 Tf
0 Tc 0 Tw T*
(\002)Tj
/T1_1 1 Tf
-0.00011 Tc 0.17841 Tw [0.1 (6\2471 integrin on lining cells is mainly due to the reduced)]TJ
0.02499 Tw T*
(proportion of the type B cells.)Tj
0.1308 Tw 1.2371 -1.2 Td
(The expression of )Tj
/T1_2 1 Tf
0 Tc 0 Tw [-0.5 (\002)]TJ
/T1_1 1 Tf
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0.099 Tw -1.2371 -1.2 Td
(was further tested in experiments in which we used enzyme)Tj
0.14709 Tw T*
(treatments to release cells from rheumatoid synovial tissue.)Tj
0.07001 Tw T*
[(The adherent synovial cells could be divided into 4 dif)17.7 (ferent)]TJ
0.15379 Tw T*
(populations, as we have shown)Tj
0.00079 Tc 0 Tw 6.3 0 0 6.5 444.3759 102.4622 Tm
(21)Tj
-0.00011 Tc 0.15379 Tw 9.7 0 0 10 450.6809 99.1621 Tm
[(. )17.8 (The elongated cells were)]TJ
-0.0304 Tw -13.6784 -1.2 Td
(positive for CD18 and CD14 and they were supposed to repre-)Tj
0.1597 Tw T*
[(sent type )54.8 (A)-354.8 (cells. )17.7 (They did not express )]TJ
/T1_2 1 Tf
0 Tc 0 Tw 16.895 0 Td
(\002)Tj
/T1_1 1 Tf
-0.00011 Tc 0.1597 Tw [0.1 (6\2471 integrin. )17.7 (The)]TJ
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1.2 TL /Im0 Do
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BT
/T1_0 1 Tf
0.27161 Tw 8 0 0 8 125 411.5293 Tm
[(Figur)36.8 (e 3.)]TJ
/T1_1 1 Tf
0.2715 Tw 4.4776 0 Td
(Subcellular localization of )Tj
/T1_2 1 Tf
0 Tc 0 Tw [-0.1 (\002)]TJ
/T1_1 1 Tf
-0.00011 Tc 0.2715 Tw [0.1 (6 integrin by immunolabeling electron microscopy on the plasma)]TJ
0.0743 Tw -4.4776 -1.25 Td
[(membrane \(arrows\) of fibroblast-like cells \(B\) \(type B cells\). )17.7 (The adjacent type )54.8 (A)-269.4 (cell \(A\) is negative \(arrow-)]TJ
0.0062 Tw 0 -1.25 TD
[(heads\). )17.8 (The nucleus and cytoplasm were counterstained lightly with uranyl acetat\
e to confirm the localization of)]TJ
0.0563 Tw T*
[(the black reaction product \(arrows\) on the plasma membrane. )17.8 (The endoplasmic reticulum has degenerated into)]TJ
0.02499 Tw T*
[(vesicular structures, because the antigenicity did not allow proper fixa\
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