Therapeutic cloning, whereby somatic cell nuclear transfer (SCNT) is used to generate
patient-specific embryonic stem cells (ESCs) from blastocysts cloned by nuclear transfer (ntESCs)…

… ranged from 12 per cent in the original report by Wakayama et al. [20] to 33 per cent in an …
, Kanagawa O& Wakayama T . 2008 Production of healthy cloned mice from bodies frozen …

Until recently, fertilization was the only way to produce viable mammalian offspring, a process
implicitly involving male and female gametes. However, techniques involving fusion of …

… Mizutani, T. Wakayama, Epigenetic abnormalities of the mouse paternal zygotic genome
associated with microinsemination of round spermatids, Dev. Biol. (2005) in press]. …

… machi R, Wakayama T. Propagation of an infertile hermaphrodite mouse lacking germ cells
… Miyake M, Wakayama T. Effect of volume of oocyte cytoplasm on embryo development after …

Embryonic stem (ES) cells are fully pluripotent in that they can differentiate into all cell types,
including gametes. We have derived 35 ES cell lines via nuclear transfer (ntES cell lines) …

Coinjection of unfertilized mouse oocytes with sperm heads and exogenous DNA encoding
either a green fluorescent protein (GFP) or β-galactosidase reporter produced 64 to 94 …

… Routine culture was on a feeder layer of mitomycin-C treated primary embryonic fibroblasts,
derived from embryonic day 13.5 mice heterozygous for the T cell antigen receptor-δ …

Specification of the germ cell lineage is vital to development and heredity. In mice, the germ
cell fate is induced in pluripotent epiblast cells by signaling molecules, yet the underlying …

Somatic stem cell depletion due to the accumulation of DNA damage has been implicated
in the appearance of aging-related phenotypes. Hair graying, a typical sign of aging in …