Practical considerations in refolding proteins from inclusion bodies

K Tsumoto, D Ejima, I Kumagai, T Arakawa - Protein expression and …, 2003 - Elsevier
… (T. Arakawa and TP Horan, unpublished results). This is due to differences in structure and
dynamics of proteins denatured by urea or guanidine HCl and by detergents, as described …

Size-exclusion chromatography with on-line light-scattering, absorbance, and refractive index detectors for studying proteins and their interactions

J Wen, T Arakawa, JS Philo - Analytical biochemistry, 1996 - Elsevier
Techniques of using size-exclusion chromatography (SEC) with on-line light-scattering, uv
absorbance, and refractive index detectors to characterize the polypeptide molecular weights …

Role of arginine in protein refolding, solubilization, and purification

…, I Kumagai, D Ejima, JS Philo, T Arakawa - Biotechnology …, 2004 - Wiley Online Library
… Recently, Tsumoto and Arakawa (79) reported that arginine affects the reversibility of
denaturation but not the melting temperature of proteins during thermal melting. Table 2 shows the …

[PDF][PDF] The stabilization of proteins by osmolytes

T Arakawa, SN Timasheff - Biophysical journal, 1985 - cell.com
The preferential interactions of lysozyme with solvent components and the effects of solvent
additives on its stability were examined for several neutral osmolytes: L-proline, L-serine, …

Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from japonica Rice

…, J Kawai, P Carninci, J Adachi, K Aizawa, T Arakawa… - science, 2003 - science.org
We collected and completely sequenced 28,469 full-length complementary DNA clones
from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly …

Empirical Analysis of Transcriptional Activity in the Arabidopsis Genome

…, JM Deng, K Akiyama, Y Ansari, T Arakawa… - science, 2003 - science.org
… Forward strand is indicated as top (T), whereas reverse strand as bottom (B). The genetically
… Mayer for gene functional category information, and T. Gingeras for support of this project. …

Cap analysis gene expression for high-throughput analysis of transcriptional starting point and identification of promoter usage

…, A Watahiki, M Nakamura, T Arakawa… - Proceedings of the …, 2003 - National Acad Sciences
We introduce cap analysis gene expression (CAGE), which is based on preparation and
sequencing of concatamers of DNA tags deriving from the initial 20 nucleotides from 5′ end …

Stabilization of protein structure by sugars

T Arakawa, SN Timasheff - Biochemistry, 1982 - ACS Publications
… where g¡ is the concentration of component z, expressed as grams of component z per
gram of water, µ,· is the chemical potential of component z, T is the thermodynamic (Kelvin) …

[HTML][HTML] An atlas of combinatorial transcriptional regulation in mouse and man

…, J Tegnér, B Lenhard, SA Teichmann, T Arakawa… - Cell, 2010 - cell.com
Combinatorial interactions among transcription factors are critical to directing tissue-specific
gene expression. To build a global atlas of these combinations, we have screened for …

[HTML][HTML] The membrane-anchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis

…, DB Alexander, C Ide, TP Horan, T Arakawa… - Cell, 2001 - cell.com
Matrix metalloproteinases (MMPs) are essential for proper extracellular matrix remodeling.
We previously found that a membrane-anchored glycoprotein, RECK, negatively regulates …