The putative oxidation of hydroethidine (HE) has become a widely used fluorescent assay
for the detection of superoxide in cultured cells. By covalently joining HE to a hexyl …

Mitochondrial dysfunction and oxidative stress contribute to motor neuron degeneration in
amyotrophic lateral sclerosis (ALS). Recent reports indicate that astrocytes expressing the …

A general protocol is described to improve the specificity for imaging superoxide formation
in live cells via fluorescence microscopy with either hydroethidine (HE) or its mitochondrially …

We report a simple method for the synthesis of peroxynitrite from nitrite and hydrogen peroxide
that can generate hundreds of milliliters of 180 mM peroxynitrite within 1 h from start to …

Nerve growth factor (NGF) can induce apoptosis by signaling through the p75 neurotrophin
receptor (p75 NTR ) in several nerve cell populations. Cultured embryonic motor neurons …

Urate is an efficient antioxidant and has recently emerged as a competitive inhibitor of tyrosine
nitration by peroxynitrite. In vivo and in vitro studies demonstrate the large extent to which …

OBJECTIVE: To determine if joint effusions encountered in the emergency department (ED)
requiring arthrocentesis possess normal lubricating ability or evidence cartilage …

Nerve growth factor (NGF) overexpression and increased production of peroxynitrite occur
in several neurodegenerative diseases. We investigated whether NGF could undergo …

A growing body of evidence supports the ‘calcium hypothesis’ of Alzheimer’s disease (AD),
which postulates that a variety of insults might disrupt the homeostatic regulation of neuronal …

Although peroxynitrite stimulates apoptosis in many cell types, whether peroxynitrite acts
directly as an oxidant or the induction of apoptosis is because of the radicals derived from …