Parkinson’s disease (PD) is a multi-attribute neurodegenerative disorder combining motor
and nonmotor symptoms without well-defined diagnostic clinical markers. The presence of …

… On average, 2% sialylation loss was observed at 50 C, 11% at 65 C, and 33% at 80 C,
suggesting that a carefully chosen derivatization temperature is crucial during glycan labeling …

… Biolabs, Ipswich, MA, USA) overnight at 37 C. The released glycans were collected from the
… ) was added to the dried sugars and incubated at 65 C for 5 hours. HPLC water (10 µL) was …

… Five microlitres labelling solution (0.37 M 12 C 6 2-AA or 0.56 M 13 C 6 2-AA containing 1 M
… glycans followed by incubation at 65 C for 5 h. C 6 -2AA and 13 C 6 -2AA labelled samples …

… 12 C/ 13 C isotopic permethylation with 12 C or 13 C methyl iodide ( 12 CH 3 I) or ( 13 CH
3 I) 169 with work also done to establish isotopic permethylation on a solid phase platform. …

… ACE activity was measured at 37 C. ACE activity measurements were performed with a
plate reader at λ ex 340 nm and λ em 405 nm (NovoStar, BMG Labtech, Ortenberg, Germany). …

… A subsequent investigation found that the separation temperature used by site A was 25C
instead of 20C, as defined in the study protocol. Due to the higher separation temperature, the …

… , 1500g centrifugation at +25 C. Sera were stored at −20 C until measurements. In case of
… taken for genetic determinations and stored at −20 C until DNA isolation. Genomic DNA was …

Cancer patients are at increased risk of developing thrombosis, comorbidity that has been
associated with increased neutrophil counts and the formation of neutrophil extracellular traps …

… was 15 μL where the sample temperature was 10 C. The column temperature was 50 C. …
The sample injection volume was 5 μL where the sample temperature was 5 C. The column …