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Anti-Alpha-Enolase Antibody as a Serologic Marker and Its Correlation with Disease Severity in Intestinal Behçet’s Disease

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Abstract

Background

Intestinal Behçet’s disease (BD) is a chronic inflammatory bowel disease, as are Crohn’s disease (CD) and ulcerative colitis (UC). But unlike CD and UC, serologic markers for intestinal BD are not well known. Recently, anti-α-enolase antibody (AAEA) has been detected in sera from BD patients.

Aims

The aim of this study was to evaluate the prevalence of AAEA in intestinal BD and its clinical correlations.

Methods

The study sample included 80 patients with intestinal BD and 23 healthy controls. IgM AAEA was detected by ELISA. The positivity of IgM AAEA was defined as an optical density greater than three standard deviations above the mean of the control sera. Other parameters, such as demographic information, subtype of BD, colonoscopic findings, disease severity and treatment modality, were analyzed retrospectively.

Results

The prevalence of IgM AAEA was 67.5% in intestinal BD and 0% in the control group. The positivity rate of IgM AAEA was higher in complete or incomplete BD than in suspected BD (77.5% vs. 51.6%, P = 0.016). The mean HBI score was higher in antibody positive patients than in antibody negative patients (5.60 vs. 4.61, P = 0.003). The cumulative probability of steroid use for aggravation of intestinal and extra-intestinal symptoms was higher in antibody positive patients than in antibody negative patients (P = 0.012). The number of patients with systemic involvement was higher in the AAEA positive group than in the negative group.

Conclusions

Monitoring IgM AAEA may be helpful for diagnosis of intestinal BD and could be used to predict clinical course and disease severity.

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References

  1. Kaklamani VG, Vaiopoulos G, Kaklamanis PG. Behçet’s disease. Semin Arthritis Rheum. 1998;27:197–217.

    Article  CAS  PubMed  Google Scholar 

  2. Sakane T, Takeno M, Suzuki N, Inaba G. Behçet’s disease. N Engl J Med. 1999;341:1284–1291.

    Article  CAS  PubMed  Google Scholar 

  3. Yurdakul S, Hamuryudan V, Yazici H. Behçet’s disease. Curr Opin Rheumatol. 2004;16:38–42.

    Article  PubMed  Google Scholar 

  4. Saxon A, Shanahan F, Landers C, Ganz T, Targan S. A distinct subset of antineutrophil cytoplasmic antibodies is associated with inflammatory bowel disease. J Allergy Clin Immunol. 1990;86:202–210.

    Article  CAS  PubMed  Google Scholar 

  5. Ruemmele FM, Targan SR, Levy G, Dubinsky M, Braun J, Seidman EG. Diagnostic accuracy of serological assays in pediatric inflammatory bowel disease. Gastroenterology. 1998;115:822–829.

    Article  CAS  PubMed  Google Scholar 

  6. Peeters M, Joossens S, Vermeire S, Vlietinck R, Bossuyt X, Rutgeerts P. Diagnostic value of anti-Saccharomyces cerevisiae and antineutrophil cytoplasmic autoantibodies in inflammatory bowel disease. Am J Gastroenterol. 2001;96:730–734.

    Article  CAS  PubMed  Google Scholar 

  7. Quinton JF, Sendid B, Reumaux D, et al. Anti-Saccharomyces cerevisiae mannan antibodies combined with antineutrophil cytoplasmic autoantibodies in inflammatory bowel disease: prevalence and diagnostic role. Gut. 1998;42:788–791.

    Article  CAS  PubMed  Google Scholar 

  8. Sendid B, Colombel JF, Jacquinot PM, et al. Specific antibody response to oligomannosidic epitopes in Crohn’s disease. Clin Diagn Lab Immunol. 1996;3:219–226.

    CAS  PubMed  Google Scholar 

  9. Adler Y, Salozhin K, Tongueze M, Shoenfeld Y, Youinou P. Anti-endothelial cell antibodies: a need for standardization. Lupus. 1994;3:77–84.

    Article  CAS  PubMed  Google Scholar 

  10. Meroni PL, Khamashta MA, Youinou P, Shoenfeld Y. Mosaic of anti-endothelial antibodies. Review of the first international workshop on anti-endothelial antibodies, clinical and pathological significance. Lupus. 1995;4:95–99.

    Article  CAS  PubMed  Google Scholar 

  11. Aydintug AO, Tokgoz G, D’Cruz DP, et al. Antibodies to endothelial cells in patients with Behçet’s disease. Clin Immunol Immunopathol. 1993;67:157–162.

    Article  CAS  PubMed  Google Scholar 

  12. Lee KH, Bang D, Choi ES, Chun WH, Lee ES, Lee S. Presence of circulating antibodies to a disease specific antigen on cultured human dermal microvascular endothelial cells in patients with Behçet’s disease. Arch Dermatol Res. 1999;291:374–381.

    Article  CAS  PubMed  Google Scholar 

  13. Lee KH, Chung HS, Kim HS, et al. Human alpha-enolase from endothelial cells as a target antigen of anti-endothelial cell antibody in Behçet’s disease. Arthritis Rheum. 2003;48:2025–2035.

    Article  CAS  PubMed  Google Scholar 

  14. Mizushima Y, Inaba G, Mimura Y. Diagnostic criteria for Behçet’s disease in 1987, and guideline for treating Behçet’s disease. Saishin Igaku. 1988;43:382–391. (in Japanese).

    Google Scholar 

  15. International Study Group for Behçet's Disease. Criteria for diagnosis of Behçet’s disease. Lancet. 1990;335:1078–1080.

    Google Scholar 

  16. Lee CR, Kim WH, Cho YS, et al. Colonoscopic findings in intestinal Behçet’s disease. Inflamm Bowel Dis. 2001;7:243–249.

    Article  CAS  PubMed  Google Scholar 

  17. Chung SY, Ha HK, Kim JH, et al. Radiologic findings of Behçet’s syndrome involving the gastrointestinal tract. Radiographics. 2001;21:911–924.

    CAS  PubMed  Google Scholar 

  18. Pratesi F, Moscato S, Sabbatini A, Chimenti D, Bombardieri S, Migliorini P. Autoantibodies specific for alpha-enolase in systemic autoimmune disorders. J Rheumatol. 2000;27:109–115.

    CAS  PubMed  Google Scholar 

  19. Pancholi V, Fischetti VA. Alpha-enolase, a novel strong plasmin(ogen) binding protein on the surface of pathogenic streptococci. J Biol Chem. 1998;273:14503–14515.

    Article  CAS  PubMed  Google Scholar 

  20. Pancholi V. Multifunctional alpha-enolase: its role in diseases. Cell Mol Life Sci. 2001;58:902–920.

    Article  CAS  PubMed  Google Scholar 

  21. Fontan PA, Pancholi V, Nociari MM, Fischetti VA. Antibodies to streptococcal surface enolase react with human alpha-enolase: implications in poststreptococcal sequelae. J Infect Dis. 2000;182:1712–1721.

    Article  CAS  PubMed  Google Scholar 

  22. Iida H, Yahara I. Yeast heat-shock protein of Mr 48,000 is an isoprotein of enolase. Nature. 1985;315:688–690.

    Article  CAS  Google Scholar 

  23. Gitlits VM, Toh BH, Sentry JW. Disease association, origin, and clinical relevance of autoantibodies to the glycolytic enzyme enolase. J Investig Med. 2001;49:138–145.

    Article  CAS  PubMed  Google Scholar 

  24. Saulot V, Vittecoq O, Charlionet R, et al. Presence of autoantibodies to the glycolytic enzyme alpha-enolase in sera from patients with early rheumatoid arthritis. Arthritis Rheum. 2002;46:1196–1201.

    Article  CAS  PubMed  Google Scholar 

  25. Roozendaal C, Zhao MH, Horst G, et al. Catalase and alpha-enolase: two novel granulocyte autoantigens in inflammatory bowel disease (IBD). Clin Exp Immunol. 1998;112:10–16.

    Article  CAS  PubMed  Google Scholar 

  26. Lee S. Diagnostic criteria of Behçet’s disease: problems and suggestions. Yonsei Med J. 1997;38:365–369.

    CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by a research grant from Ferring Korea.

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Authors and Affiliations

  1. Department of Internal Medicine, Institute of Gastroenterology, Yonsei University College of Medicine, 134 Shinchon-Dong Seodaemun-ku, Seoul, 120-752, Korea

    Sung Jae Shin, Byung Chang Kim, Tae Il Kim, Sang Kil Lee & Won Ho Kim

  2. Department of Dermatology, Yonsei University College of Medicine, 134 Shinchon-Dong Seodaemun-ku, Seoul, 120-752, Korea

    Kwang Hoon Lee

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  1. Sung Jae Shin
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  2. Byung Chang Kim
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  3. Tae Il Kim
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  4. Sang Kil Lee
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  5. Kwang Hoon Lee
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  6. Won Ho Kim
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Correspondence to Won Ho Kim.

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Shin, S.J., Kim, B.C., Kim, T.I. et al. Anti-Alpha-Enolase Antibody as a Serologic Marker and Its Correlation with Disease Severity in Intestinal Behçet’s Disease. Dig Dis Sci 56, 812–818 (2011). https://doi.org/10.1007/s10620-010-1326-y

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  • DOI: https://doi.org/10.1007/s10620-010-1326-y

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