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Tumor necrosis factor-alpha and Interleukin-10 gene promoter polymorphisms in Turkish rheumatoid arthritis patients

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Abstract

Tumor necrosis factor and interleukin 10 have been implicated in the pathogenesis of rheumatoid arthritis (RA). Certain single-nucleotide polymorphisms (SNPs) within the promoter region of the IL-10 and TNF genes have been associated with altered levels of circulating IL10 and TNF. We aimed to explore the association of IL-10 and TNF-α polymorphisms in Turkish RA patients. We analyzed the association of TNF-α (−308G/A, −238G/A, −376G/A) and IL10 (−1082G/A, −819C/T, −592C/A) polymorphisms in 98 Turkish patients with rheumatoid arthritis and 122 healthy subjects using ARMS-PCR. The correlation of these findings with RF positivity and erosive disease in RA patients was also sought. A significant association was found between having RA and −1082 G allele (p = 0.008; OR = 1.44, 95% CI 1.11–1.86). There was no association between RA and −819C/T polymorphism. Significant differences were observed in IL10 GCC and ACC haplotypes distribution between RA and control subjects (p = 0.006; OR = 1.46, 95% CI 1.13–1.89 and p = 0.011; OR = 1.43, 95% CI 1.09–1.88, respectively). No statistically significant association was found between TNF-α 308G/A, −238G/A, −376G/A polymorphisms and RA. No significant association was found between RF positivity and erosive disease and TNF-α, IL10 gene polymorphisms. In addition, when combined genotypes were analyzed, no significant difference was found between RA patients and healthy controls. Our findings suggest that IL-10 1082 G/A polymorphism or GCC, ACC haplotypes may be associated with RA in Turkish patients.

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Acknowledgment

The authors thank Hasan Yazici for the critical review of the manuscript.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Science Faculty, Istanbul University, 34118 Vezneciler, Istanbul, Turkey

    Omer Ates & Aysegul Topal-Sarikaya

  2. Rheumatology Department, Cerrahpasa Medical Faculty, Istanbul University, Istanbul, Turkey

    Gulen Hatemi & Vedat Hamuryudan

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  1. Omer Ates
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  2. Gulen Hatemi
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  3. Vedat Hamuryudan
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  4. Aysegul Topal-Sarikaya
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Correspondence to Omer Ates.

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Ates, O., Hatemi, G., Hamuryudan, V. et al. Tumor necrosis factor-alpha and Interleukin-10 gene promoter polymorphisms in Turkish rheumatoid arthritis patients. Clin Rheumatol 27, 1243–1248 (2008). https://doi.org/10.1007/s10067-008-0893-1

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  • DOI: https://doi.org/10.1007/s10067-008-0893-1

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