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Antioxidant status, lipid peroxidation and nitric oxide in fibromyalgia: etiologic and therapeutic concerns

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Abstract

We proposed to assess the oxidant/antioxidant status, lipid peroxidation and nitric oxide (NO) in untreated fibromyalgia (FM) patients and controls. The effect of amitriptyline (A, 20 mg daily) and sertraline (S, 100 mg daily) treatment on patients’ superoxide dismutase (SOD), xanthine oxidase (XO), adenosine deaminase (ADA) enzyme activities, thiobarbituric acid reactive substances (TBARS) and NO levels was investigated. Thirty female patients with primary FM and age-matched 16 healthy female controls were included. Patients received an 8-week course of treatment with either A or S. FM patients had higher serum levels of TBARS (particularly malondialdehyde) and lower levels of nitrite compared to controls whereas enzyme activities were similar. A and S significantly improved Fibromyalgia Impact Questionnaire (FIQ) pain scores, Hamilton anxiety and depression rating scales. But neither A nor S had significant effects on measured oxidative stress parameters, except SOD activity that was significantly reduced after S treatment. Total myalgic scores negatively correlated with XO activity, and depression scales negatively correlated with levels of TBARS. Our results indicate that patients with FM are under oxidative stress. These findings represent a rationale for further research assessing the effect of free radical scavengers or antioxidant agents like vitamins and omega-3 fatty acids on peripheral and central mechanisms in FM.

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Abbreviations

FM:

Fibromyalgia

NO:

Nitric oxide

S:

Sertraline

A:

Amitriptyline

SOD:

Superoxide dismutase

XO:

Xanthine oxidase

ADA:

Adenosine deaminase

TBARS:

Thiobarbituric acid reactive substances

FIQ:

Fibromyalgia Impact Questionnaire

ROS:

Reactive oxygen species

CFS:

Chronic fatigue syndrome

O2 :

Dioxygen

NOS:

Nitric oxide synthase

PPT:

Pain pressure threshold

HDRS:

Hamilton Depression Rating Scale

HARS:

Hamilton Anxiety Rating Scale

PUFAs:

Polyunsaturated fatty acids

NBT:

Nitroblue tetrazolium

PDE:

Phosphodiesters

MDA:

Malondialdehyde

NO −2 :

Nitrite

NO. :

Nitric oxide radical

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Authors and Affiliations

  1. Division of Rheumatology, Department of Physical Medicine and Rehabilitation, Faculty of Medicine, Firat University, 23119, Elazig, Turkey

    Salih Ozgocmen, Ozge Ardicoglu & Hulya Yildizhan

  2. Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey

    Huseyin Ozyurt

  3. Department of Biochemistry, Faculty of Medicine, Mustafa Kemal University, Antakya, Turkey

    Sadik Sogut

  4. Department of Biochemistry, School of Medicine, Hacettepe University, Ankara, Turkey

    Omer Akyol

Authors
  1. Salih Ozgocmen
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  2. Huseyin Ozyurt
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  3. Sadik Sogut
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  4. Omer Akyol
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  6. Hulya Yildizhan
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Correspondence to Salih Ozgocmen.

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Ozgocmen, S., Ozyurt, H., Sogut, S. et al. Antioxidant status, lipid peroxidation and nitric oxide in fibromyalgia: etiologic and therapeutic concerns. Rheumatol Int 26, 598–603 (2006). https://doi.org/10.1007/s00296-005-0079-y

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