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Immunoelectron microscopic localization of HLA-DR antigen in control small intestine and colon and in inflammatory bowel disease

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Abstract

We have elucidated the distribution of I2 (HLA-DR) antigen in control and inflammatory bowel disease specimens, using immunoelectron microscopic methods. Control small intestinal epithelium and inflammatory bowel disease epithelium expressed I2 antigen, while control colonic epithelium did not. I2 expression by enterocytes was more frequent on the lateral and basal surface than on the microvillus surface. Two of three M cells in control ileum expressed I2 antigen. I2-positive intraepithelial lymphocytes were rarely detected in both control and disease specimens. I2-positive lamina propria lymphocytes were significantly increased in inflammatory bowel disease, while I2-positive lamina propria lymphocytes were virtually absent in control specimens. I2-positive mononuclear cells in the intestinal lamina propria were largely macrophages and monocytes in both control and inflammatory bowel disease specimens. I2-positive mononuclear cells resembling dendritic cells were not detected in control or disease specimens. Furthermore, there were no significant morphological differences in I2-positive or-negative macrophages and monocytes in control and disease specimens. The expression of I2 antigen on Schwann cells was detected more frequently in disease specimens than in control specimens. Capillary endothelia of both control and disease specimens expressed I2 antigen. We demonstrate that I2 expression is present on surface membranes of both immune and nonimmune cells of the intestine and colon and show that this expression is more prominent in inflammatory bowel disease than in control intestine and colon. Further studies are required to determine whether this finding is meaningful in terms of antigen presentation and whether this apparent “immune activation” is involved in the pathogenesis of inflammatory bowel disease.

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Author notes

  1. Ichiro Hirata MD

    Present address: The 2nd Division of Internal Medicine, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki Osaka, Japan

Authors and Affiliations

  1. Departments of Internal Medicine, University of Michigan Medical School, 2215 Fuller Road, 48105, Ann Arbor, Michigan

    Ichiro Hirata MD, Linda L. Austin BA, Walter H. Blackwell BA, John R. Weber BS &  William O. Dobbins III MD

  2. Veterans Administration Medical Center, 2215 Fuller Road, 48105, Ann Arbor, Michigan

    Ichiro Hirata MD, Linda L. Austin BA, Walter H. Blackwell BA, John R. Weber BS &  William O. Dobbins III MD

Authors
  1. Ichiro Hirata MD
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  2. Linda L. Austin BA
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  3. Walter H. Blackwell BA
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  4. John R. Weber BS
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  5. William O. Dobbins III MD
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Supported by funding from the National Foundation for Ileitis and Colitis and by Merit Review funds from the Veterans Administration.

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Hirata, I., Austin, L.L., Blackwell, W.H. et al. Immunoelectron microscopic localization of HLA-DR antigen in control small intestine and colon and in inflammatory bowel disease. Digest Dis Sci 31, 1317–1330 (1986). https://doi.org/10.1007/BF01299810

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  • DOI: https://doi.org/10.1007/BF01299810

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