Kinetics of Asian and African ZIKV replication in HOBIT cells. Supernatants of HOBIT infected with either ZIKV strain at the indicated MOI were collected at the indicated timepoints and back-titrated on Vero E6 cell line by limiting dilution assay. Infectious viral titers were expressed as log tissue culture infectious dose (TCID)50/ml.
| ZIKV Strain | MOI (TCID50/cells) | Virus Yield (Log TCID50/ml) | ||||
|---|---|---|---|---|---|---|
| 6 hpi | 24 hpi | 48 hpi | 72 hpi | 96 hpi | ||
| PRVABC59 (Asian) | 0.1 | < 1.3 | 3.6 | 5.6 | 6.3 | 6.9 |
| 1 | 1.9 | 5.3 | 6.2 | 6.8 | 7.3 | |
| MR766 (African) | 0.1 | < 1.3 | 3.8 | 6 | 7.8 | 7.8 |
| 1 | < 1.3 | 5.0 | 7.3 | 7.8 | 7.9 | |
ZIKV: Zika virus; HOBIT: human osteoblast-like SV-40 T antigen immortalized cell line; MOI: multiplicity of infection; hpi: hours postinfection.