Sequence of the primers used for amplifying exons 2 and 3 of the HLA-B27 gene and SNP in IL-23R and ERAP1 genes, together with the annealing temperature (T) corresponding to each pair of primers and the size of the amplification product obtained.
| Primer | Primer Sequence (5′ to 3′) | T (ºC) | Length of Amplification (pb) |
|---|---|---|---|
| HLAB2-1F* | GCC GCG AGT CCG AGA GA (17) | 65 | 117 |
| HLAB2-1R* | GGC CTC GCT CTG GTT GTA (18) | ||
| HLAB2-2F | CCG GAG TAT TGG GAC CG (17) | 65 | 67 |
| HLAB2-2R | GGC CTC GCT CTG GTT GTA G (19) | ||
| HLAB3-2F | GGG CAG GGT CTC ACA CCC TCC (21) | 65 | 61 |
| HLAB3-2R | GAT GTA ATC CTT GCC GTC GTA (21) | ||
| HLAB3-3F | GGA TTA CAT CGC CCT GAA CG (20) | 60 | 92 |
| HLAB3-3R | TCC ACG CAC TCG CCC TCC AGG T (22) | ||
| IL23R-rs2201841-F | GTG ATG ATT TGT GAC AGT AGT A (22) | 60 | 69 |
| IL23R-rs2201841-R | AAG TGC TGG GCT TAC AGG CA (20) | ||
| IL23R-rs11209026-F | CTT TGA TTG GGA TAT TTA AC (20) | 55 | 86 |
| IL23R-rs11209026-R | CAT ATA CAT GTA GTC TAA ATC AG (23) | ||
| IL23R-rs11209032-F | GCT ATC CTG ACA ATT CCT C (19) | 53 | 83 |
| IL23R-rs11209032-R | CTT CAA GCT GAA TTG CA (17) | ||
| ERAP1-rs2287987-F | ATG AGC TTA TAC CTG GTG AGC (21) | 60 | 32 |
| ERAP1-rs2287987-R | AGT CTT CTG CAT CAA GTA AGC (21) | ||
| ERAP1-rs30187-F | GCT CTT GCT TCA TGT GTA CA (20) | 62 | 87 |
| ERAP1-rs30187-R | CCA TGA TGA ACA CTT GGA CAC (21) | ||
| ERAP1-rs27044-F | AGC CTT CTG CCC TCT GTA (18) | 58 | 110 |
| ERAP1-rs27044-R | GCA GAC ATG GAC AGA CGA G (19) |
↵* Primer from Dominguez, et al, Immunogenetics, 1992. SNP: single-nucleotide polymorphism; IL: interleukin.