Indirect immunofluorescence and specificity of ANCA testing in the 22 gram-positive endocarditis group.
| ANCA | |||
|---|---|---|---|
| Endocarditis Pathogen | IIF | ELISA (normal < 20 IU) | |
| MPO | PR3 | ||
| Staphylococcus aureus | — | 3 | 1 |
| S. aureus | Cytoplasmic+ | 3 | 2 |
| S. aureus | — | 5 | 1 |
| S. aureus | — | 3 | 1 |
| Staphylococcus epidermidis | — | 3 | 1 |
| S. aureus | — | 3 | 1 |
| S. aureus | Cytoplasmic+ | 3 | 2 |
| S. aureus | — | 4 | 3 |
| S. aureus | — | 4 | 3 |
| S. aureus | Cytoplasmic+ | 9 | 28 |
| S. aureus | — | 3 | 2 |
| Streptococcus sanguinis | — | 3 | 2 |
| Streptococcus mitis | — | 2 | 1 |
| S. mitis | Cytoplasmic+ | 2 | 1 |
| Streptococcus gordonii | — | 4 | 5 |
| Streptococcus dysgalactiae | — | 3 | 1 |
| S. sanguinis | — | 3 | 2 |
| S. mitis | — | 3 | 1 |
| Enterococcus faecalis | — | 3 | 2 |
| E. faecalis | — | 3 | 2 |
| E. faecalis | — | 2 | 3 |
| E. faecalis | Cytoplasmic+ | 3 | 5 |
Cytoplasmic + refers to a positive fluorescence (titers about 40 IU/ml), and cytoplasmic ++ refers to a high positive fluorescence (titers about 160 IU/ml). ANCA: antineutrophil cytoplasmic antibodies; IIF: indirect immunofluorescence; MPO: myeloperoxidase; PR3: proteinase 3.