Oligonucleotide primers used for PCR and expected template size.
| Target Gene | Forward Primer (5′ → 3′) Reverse Primer (5′ → 3′) | Expected Template Size (base pair) |
|---|---|---|
| TLR 2 | GGC CAG CAA ATT ACC TGT GTG AGG CGG ACA TCC TGA ACC T | 69 |
| TLR 4 | TCC ATA AAA GCC GAA AGG TG CTG AGC AGG GTC TTC TCC AC | 195 |
| TLR 5 | TGC CTT GAA GCC TTC AGT TAT G CCA ACC ACC ACC ATG ATG AG | 75 |
| TLR 9 | GCT AGA CCT GTC CCG CAA TA ACA CTT GGC TGT GGA TGT TG | 196 |
| ß-actin | TCA CCC ACA CTG TGC CCA TCT ACG A CAG CGG AAC CGC TCA TTG CCA ATG G | 295 |