RT Journal Article SR Electronic T1 Lack of association of ankylosing spondylitis with the most common NOD2 susceptibility alleles to Crohn's disease. JF The Journal of Rheumatology JO J Rheumatol FD The Journal of Rheumatology SP 102 OP 104 VO 30 IS 1 A1 Isabel Ferreirós-Vidal A1 Juan Amarelo A1 Francisco Barros A1 Angel Carracedo A1 Juan J Gómez-Reino A1 Antonio Gonzalez YR 2003 UL http://www.jrheum.org/content/30/1/102.abstract AB OBJECTIVE: To investigate whether the 3 most common mutations in the NOD2 gene that confer susceptibility to Crohn's disease (CD) are also associated with ankylosing spondylitis (AS). METHODS: DNA from 112 patients with AS and 168 controls of homogenous Spanish ancestry were studied. The frequencies of the pathogenic alleles of NOD2 (3020insC, 2722G>C, and 2104C>T) were determined by analysis of the melting curves after hybridization with FRET probes on a Light Cycler real-time polymerase chain reaction (PCR) system. Results. NOD2 allelic frequencies in controls (3020insC, 0.009; 2722G>C, 0.009; 2104C>T, 0.042) did not significantly differ from patients with AS (3020insC, 0.009; 2722G>C, 0.004; 2104C>T, 0.031). CONCLUSION: The 3 most common CD NOD2 mutations do not contribute to disease susceptibility to AS, and therefore do not explain the susceptibility locus for AS in chromosome 16q.