RT Journal Article
SR Electronic
T1 Autoantibodies to Dense Fine Speckles in Pediatric Diseases and Controls
JF The Journal of Rheumatology
JO J Rheumatol
FD The Journal of Rheumatology
SP jrheum.150567
DO 10.3899/jrheum.150567
A1 Heinrike Schmeling
A1 Michael Mahler
A1 Deborah M.  Levy
A1 Katharine Moore
A1 Anne M.  Stevens
A1 James Wick
A1 Jacob D.  McMillan
A1 Gerd Horneff
A1 Shervin Assassi
A1 Julio Charles
A1 Gloria Salazar
A1 Maureen D.  Mayes
A1 Earl D.  Silverman
A1 Marissa Klien-Gitelman
A1 Tzelan Lee
A1 Hermine I.  Brunner
A1 Ann M.  Reed
A1 Marvin J.  Fritzler
YR 2015
UL http://www.jrheum.org/content/early/2015/10/07/jrheum.150567.abstract
AB Objective Autoantibodies to the dense fine speckled 70 kDa antigen (DFS70) are reported to be more common in individuals who do not have an antinuclear antibody (ANA)-associated rheumatic disease (AARD) than in patients with AARD. The frequency of anti-DFS70 antibodies has been thoroughly studied in adult but not in pediatric populations. The primary objective of this observational study was to determine the frequency of anti-DFS70 in pediatric AARD and reference cohorts. Methods Sera from 743 children with AARD and related conditions, and 345 samples from reference cohorts (healthy children and those being investigated for AARD) were studied for anti-DFS70 autoantibodies as measured by a chemiluminescence immunoassay. A de-identified administrative database was used to retrieve demographic, serologic, and clinical data. Results Anti-DFS70 antibodies were seen in 2.1% of healthy children and in 4.5% of sera from pediatric individuals referred for ANA testing. The frequency of anti-DFS70 was highest in juvenile localized scleroderma (LS; 4/29, 13.8%), juvenile dermatomyositis (JDM; 2/11, 18.2%), childhood systemic lupus erythematosus (cSLE; 19/331, 5.7%), diffuse cutaneous systemic sclerosis (1/22, 4.5%), celiac disease (2/49, 4.1%), and juvenile idiopathic arthritis (JIA; 5/202, 2.5%). Of note, anti-DFS70 antibodies were observed in 3/26 children (11.5%) with uveitis and JIA-associated uveitis. Conclusion The frequency of anti-DFS70 autoantibodies in healthy pediatric subjects is within the lower range of that reported in adults. Anti-DFS70 antibodies can be found in childhood SSc and cSLE, but has a remarkably high frequency in children with LS, JDM, and uveitis.