PT - JOURNAL ARTICLE AU - Norma Lucena-Silva AU - Veridiana Sales Barbosa de Souza AU - Renan Garcia Gomes AU - Alex Fantinatti AU - Yara Costa Netto Muniz AU - Rafael Sales de Albuquerque AU - Alessandra Luna Ramos Monteiro AU - George Tadeu Nunes Diniz AU - Maria Rosângela Cunha Duarte Coelho AU - Celso Teixeira Mendes-Junior AU - Erick da Cruz Castelli AU - Eduardo Antônio Donadi TI - HLA-G 3' Untranslated Region Polymorphisms Are Associated with Systemic Lupus Erythematosus in 2 Brazilian Populations AID - 10.3899/jrheum.120814 DP - 2013 May 15 TA - The Journal of Rheumatology PG - jrheum.120814 4099 - http://www.jrheum.org/content/early/2013/05/13/jrheum.120814.short 4100 - http://www.jrheum.org/content/early/2013/05/13/jrheum.120814.full AB - Objective HLA-G has well recognized tolerogenic properties in physiological and nonphysiological conditions. The 3’ untranslated region (3’UTR) of the HLA-G gene has at least 3 polymorphic sites (14-bpINS/DEL, +3142C/G, and +3196C/G) described as associated with posttranscriptional influence on messenger RNA production; however, only the 14-bpINS/DEL and +3142C/G sites have been studied in systemic lupus erythematosus (SLE). Methods We investigated the HLA-G 3’UTR polymorphic sites (14-bpINS/DEL, +3003C/T, +3010C/G, +3027A/C, +3035C/T, +3142C/G, +3187A/G, and +3196C/G) in 190 Brazilian patients with SLE and 282 healthy individuals in allele, genotype, and haplotype analyses. A multiple logistic regression model was used to assess the association of the disease features with the HLA-G 3’UTR haplotypes. Results Increased frequencies were observed of the 14-bpINS (p = 0.053), +3010C (p = 0.008), +3142G (p = 0.006), and +3187A (p = 0.013) alleles, and increased frequencies of the 14-bpINS-INS (p = 0.094), +3010 C-C (p = 0.033), +3142 G-G (p = 0.021), and +3187 A-A (p = 0.035) genotypes. After Bonferroni correction, only the +3142G (p = 0.05) and +3010C (p = 0.06) alleles were overrepresented in SLE patients. The UTR-1 haplotype (14-bpDEL/+3003T/+3010G/+3027C/+3035C/+3142C/+3187G/+3196C) was underrepresented in SLE (pcorr = 0.035). Conclusion These results indicate that HLA-G 3’UTR polymorphic sites, particularly +3142G and +3010C alleles, were associated with SLE susceptibility, whereas UTR-1 was associated with protection against development of SLE.