PT - JOURNAL ARTICLE AU - Ehtisham Akhter AU - Zakera Shums AU - Gary L. Norman AU - Walter Binder AU - Hong Fang AU - Michelle Petri TI - Utility of Antiphosphatidylserine/Prothrombin and IgA Antiphospholipid Assays in Systemic Lupus Erythematosus AID - 10.3899/jrheum.120084 DP - 2013 Feb 01 TA - The Journal of Rheumatology PG - jrheum.120084 4099 - http://www.jrheum.org/content/early/2013/01/27/jrheum.120084.short 4100 - http://www.jrheum.org/content/early/2013/01/27/jrheum.120084.full AB - Objective Currently, 3 antiphospholipid assays are widely used clinically [lupus anticoagulant (LAC), anticardiolipin (aCL), and anti-ß2-glycoprotein I (anti-ß2-GPI)]. LAC is the most specific assay, conferring the highest risk of thrombosis and pregnancy loss, but it cannot be validly performed in an anticoagulated patient. We investigated the usefulness of antiphosphatidyl-serine/prothrombin (anti-PS/PT) and its association with thrombosis. Anti-PS/PT is strongly associated with the presence of LAC. We also studied the association of IgA antiphospholipid isotypes and specific domains of ß2-GPI with thrombosis in systemic lupus erythematosus (SLE). Methods Stored samples from patients with SLE, with and without past thrombosis, were assayed for antibodies to the whole ß2-GPI protein (IgG/IgM/IgA), to ß2-GPI domain 1 (IgG), to ß2-GPI domain 4/5 (IgA), aCL (IgG/IgM/IgA), and anti-PS/PT (IgG, IgM, and IgG/M). LAC was detected using the dilute Russell’s viper venom time (dRVVT) with confirmatory testing. Results Anti-PS/PT IgG and IgG/M and anti-ß2-GPI IgG, IgM, and IgA were highly associated with a history of LAC by dRVVT (p < 0.0001). For all thrombosis, of the traditional ELISA assays, anti-ß2-GPI IgA, IgG, and aCL IgA were most associated. Anti-PS/PT IgG and IgG/M had a similar magnitude of association to the traditional ELISA. For venous thrombosis, of the traditional ELISA, anti-ß2-GPI (IgG and IgA), anti-PS/PT (IgG and IgG/M), and aCL IgA were associated. Again, anti- PS/PT (IgG and IgG/M) had the same magnitude of association as the traditional ELISA. For stroke, significant association was seen with anti-ß2-GPI IgA D4/5. Conclusion In anticoagulated patients, where LAC testing is not valid, anti-PS/PT, either IgG or IgG/IgM, might serve as useful alternative tests to predict a higher risk of thrombosis. Anti-PS/PT antibodies were associated with all thrombosis and with venous thrombosis. IgA isotypes in secondary antiphospholipid syndrome are associated with thrombosis. Anti-ß2-glycoprotein domain 1 was not shown to be associated with thrombosis in SLE.