TY - JOUR T1 - Preferential Accumulation of Activated Th1 Cells Not Only in Rheumatoid Arthritis But Also in Osteoarthritis Joints JF - The Journal of Rheumatology JO - J Rheumatol DO - 10.3899/jrheum.101355 SP - jrheum.101355 AU - Hisakata Yamada AU - Yasuharu Nakashima AU - Ken Okazaki AU - Taro Mawatari AU - Jun-Ichi Fukushi AU - Akiko Oyamada AU - Kenjiro Fujimura AU - Yukihide Iwamoto AU - Yasunobu Yoshikai Y1 - 2011/05/01 UR - http://www.jrheum.org/content/early/2011/04/25/jrheum.101355.abstract N2 - Objective It was previously found that Th1 but not Th17 cells were predominant in the joints of rheumatoid arthritis (RA). To verify whether this is a unique feature of CD4 T cells in RA joints, we performed comparative flow cytometric analysis of CD4 T cells in RA and osteoarthritis (OA) joints. Methods Mononuclear cells were isolated from peripheral blood (PB), synovial membrane (SM), and synovial fluid (SF) from a total of 18 RA and 12 OA patients. The expression of surface molecules and cytokine production of CD4 T cells was examined by a flow cytometer. Results Most CD4 T cells in RA joints expressed memory/activation markers, such as CD45RO, HLA-DR, and CD69. CCR5 was highly expressed on CD4 T cells in SF but not in PB or SM. With regard to Th17-related molecules, CD4 T cells expressing CCR6 were not enriched in either SF or SM. In contrast, CD161-positive cells were abundant in the joint, many of which, however, produced interferon-γ but not interleukin 17A. Virtually all T cells in OA joints, although much less numerous than in RA joints, expressed activation markers. Th1 cells were predominant in both OA and RA joints, while there were a few Th17 cells. The frequency of Th17 cells in the joint tended to be lower in OA than RA. Conclusion There was a quantitative but not qualitative difference in CD4 T cells, including the expression of activation markers and cytokine profiles, between RA and OA joints. ER -