@article {Paz{\'a}rjrheum.090806, author = {Borb{\'a}la Paz{\'a}r and Enik{\H o} S{\'a}fr{\'a}ny and P{\'e}ter Gergely and S{\'a}ndor Sz{\'a}nt{\'o} and Zolt{\'a}n Szekanecz and Gyula Po{\'o}r}, title = {Association of ARTS1 Gene Polymorphisms with Ankylosing Spondylitis in the Hungarian Population: The rs27044 Variant Is Associated with HLA-B*2705 Subtype in Hungarian Patients with Ankylosing Spondylitis}, elocation-id = {jrheum.090806}, year = {2009}, doi = {10.3899/jrheum.090806}, publisher = {The Journal of Rheumatology}, abstract = {Objective Associations have been found between ankylosing spondylitis (AS) and polymorphisms in the aminopeptidase regulator of TNFR1 shedding (ARTS1) gene. We studied the association of 5 polymorphisms within the ARTS1 gene with AS in Hungarian patients. We also investigated the prevalence of HLA-B27 subtypes in the Hungarian population. Methods A case-control study including 297 patients with AS and 200 sex and ethnically matched healthy controls was performed. Patients and controls were genotyped for rs27044, rs17482078, rs10050860, rs30187, and rs2287987 single-nucleotide polymorphisms using real-time polymerase chain reaction (PCR) allelic discrimination. HLA-B27 subtypes were determined with PCR sequence-specific primer (PCR-SSP) technique. Results We observed a significant increase in the minor allele frequency of rs27044 (p = 0.001) in the AS group compared to controls. The minor allele frequencies of rs10050860 (p = 0.006) and rs2287987 (p = 0.002) showed a significant decrease inAS patients compared to controls. Haplotype analysis revealed association of 2 ARTS1 haplotypes with AS in the Hungarian population. We found that HLA-B*2705 was the predominant subtype in Hungarians with AS. Carriage of the G allele of rs27044 was significantly associated with the HLA-B*2705 subtype (p = 0.009) in AS patients. Conclusion We confirmed reported associations of ARTS1 gene polymorphisms with AS in a Hungarian cohort study. We found HLA-B*2705 as the predominant subtype in Hungarian AS patients in accord with other studies on Caucasian populations. Our results suggest that the ARTS1 gene variants together with HLA-B27 strongly contribute to disease susceptibility in patients with AS.}, issn = {0315-162X}, URL = {https://www.jrheum.org/content/early/2009/12/21/jrheum.090806}, eprint = {https://www.jrheum.org/content/early/2009/12/21/jrheum.090806.full.pdf}, journal = {The Journal of Rheumatology} }