RT Journal Article
SR Electronic
T1 Effect of Blockade of Tumor Necrosis Factor-α with Etanercept on Surgical Wound Healing in SWISS-OF1 Mice
JF The Journal of Rheumatology
JO J Rheumatol
FD The Journal of Rheumatology
SP jrheum.081285
DO 10.3899/jrheum.081285
A1 Estibaliz Iglesias
A1 Francisco O´Valle
A1 Juan Salvatierra
A1 Jose Aneiros-Fernández
A1 Jesus Cantero-Hinojosa
A1 Pedro Hernández-Cortés
YR 2009
UL http://www.jrheum.org/content/early/2009/08/29/jrheum.081285.abstract
AB Objective To assess whether blockade of tumor necrosis factor-α (TNF-α) influences surgical wound healing in a normal mouse experimental model. Methods Wound healing time course and degree of surgical wound collagenization were measured by morphological techniques and digital image analysis in 80 male SWISS-OF1 mice (40 received subcutaneous etanercept at a dose of 0.1 mg/25g/ at -7, 0, 7, and 14 days). Results No significant differences were observed between treated and untreated animals in wound healing, re-epithelialization, or formation of inflammatory infiltrate or granulation tissue at days 7, 15, or 20 after surgery. At 20 days, the collagen area was larger in treated versus untreated mice (109029 ± 28489 µm2 vs 79305 ± 19798 µm2, p = 0.026, Mann-Whitney U test). Conclusion Surgical wounds showed a higher degree of collagenization at 20 days in etanercept-treated versus untreated mice, with no differences in the time course of wound healing. These data suggest that biological therapies to block TNF-α do not affect wound healing and do not need to be suspended during the perioperative period.