RT Journal Article
SR Electronic
T1 HLA-G 3′ Untranslated Region Polymorphisms Are Associated with Systemic Lupus Erythematosus in 2 Brazilian Populations
JF The Journal of Rheumatology
JO J Rheumatol
FD The Journal of Rheumatology
SP 1104
OP 1113
DO 10.3899/jrheum.120814
VO 40
IS 7
A1 Norma Lucena-Silva
A1 Veridiana Sales Barbosa de Souza
A1 Renan Garcia Gomes
A1 Alex Fantinatti
A1 Yara Costa Netto Muniz
A1 Rafael Sales de Albuquerque
A1 Alessandra Luna Ramos Monteiro
A1 George Tadeu Nunes Diniz
A1 Maria Rosângela Cunha Duarte Coelho
A1 Celso Teixeira Mendes-Junior
A1 Erick da Cruz Castelli
A1 Eduardo Antônio Donadi
YR 2013
UL http://www.jrheum.org/content/40/7/1104.abstract
AB Objective. HLA-G has well recognized tolerogenic properties in physiological and nonphysiological conditions. The 3′ untranslated region (3′UTR) of the HLA-G gene has at least 3 polymorphic sites (14-bpINS/DEL, +3142C/G, and +3196C/G) described as associated with posttranscriptional influence on messenger RNA production; however, only the 14-bpINS/DEL and +3142C/G sites have been studied in systemic lupus erythematosus (SLE). Methods. We investigated the HLA-G 3′UTR polymorphic sites (14-bpINS/DEL, +3003C/T, +3010C/G, +3027A/C, +3035C/T, +3142C/G, +3187A/G, and +3196C/G) in 190 Brazilian patients with SLE and 282 healthy individuals in allele, genotype, and haplotype analyses. A multiple logistic regression model was used to assess the association of the disease features with the HLA-G 3′UTR haplotypes. Results. Increased frequencies were observed of the 14-bpINS (p = 0.053), +3010C (p = 0.008), +3142G (p = 0.006), and +3187A (p = 0.013) alleles, and increased frequencies of the 14-bpINS-INS (p = 0.094), +3010 C-C (p = 0.033), +3142 G-G (p = 0.021), and +3187 A-A (p = 0.035) genotypes. After Bonferroni correction, only the +3142G (p = 0.05) and +3010C (p = 0.06) alleles were overrepresented in SLE patients. The UTR-1 haplotype (14-bpDEL/+3003T/+3010G/+3027C/+3035C/+3142C/+3187G/+3196C) was underrepresented in SLE (pcorr = 0.035). Conclusion. These results indicate that HLA-G 3′UTR polymorphic sites, particularly +3142G and +3010C alleles, were associated with SLE susceptibility, whereas UTR-1 was associated with protection against development of SLE.