%0 Journal Article %A HUI SHEN %A JANE C. GOODALL %A J.S. HILL GASTON %T Frequency and Phenotype of T Helper 17 Cells in Peripheral Blood and Synovial Fluid of Patients with Reactive Arthritis %D 2010 %R 10.3899/jrheum.100146 %J The Journal of Rheumatology %P 2096-2099 %V 37 %N 10 %X Objective. To analyze the frequency and phenotype of peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cell (SFMC) T helper (Th)17 cells in reactive arthritis (ReA). Methods. T cell surface phenotype and cytokine production were measured following stimulation, using 8-color flow cytometry. Results. The percentages of interleukin 17 (IL-17)–positive CD4+ T cells were increased in SFMC of patients with ReA compared with PBMC. All IL-17+ cells were CD4+CD45RO+; in SFMC most expressed CCR6, but only 50% expressed CCR4. IL-17+ cells sometimes coexpressed IL-22 and/or interferon-γ, but not IL-10. Conclusion. These data support the hypothesis that Th17 cells are involved in ReA pathogenesis. %U https://www.jrheum.org/content/jrheum/37/10/2096.full.pdf