TY - JOUR T1 - Cyclooxygenase-2 polymorphisms and risk of rheumatoid arthritis in Koreans. JF - The Journal of Rheumatology JO - J Rheumatol SP - 763 LP - 769 VL - 35 IS - 5 AU - Hye-Ryeon Yun AU - Soo-Ok Lee AU - Eun Ju Choi AU - Hyoung Doo Shin AU - Jae-Bum Jun AU - Sang-Cheol Bae Y1 - 2008/05/01 UR - http://www.jrheum.org/content/35/5/763.abstract N2 - OBJECTIVE: To determine the association of single-nucleotide polymorphisms (SNP) in the cyclooxygenase-2 (COX-2) gene with the risk and radiologic severity of rheumatoid arthritis (RA) in Koreans. METHODS: Sequencing of the COX-2 gene using a DNA analyzer revealed genetic variants in 24 Korean DNA samples. A total of 1201 Korean patients with RA and 973 controls were genotyped using the TaqMan method. HLA-DRB1 was genotyped by polymerase chain reaction and sequence-specific oligonucleotide probe hybridization techniques. Logistic regression models were used to calculate odds ratios (OR) and 95% confidence intervals (95% CI) and the corresponding probability values for each SNP site and haplotype. RESULTS: Direct sequencing identified 23 SNP of COX-2 gene, from which 2 common SNP (-1329A-->G and 6365T-->C) were selected based on the linkage disequilibrium status among SNP and minor allele frequencies. The -899G-->C SNP was also studied because it is reportedly associated with the risk of RA. The -1329A-->G SNP was not significantly associated with the risk of RA. However, the risk of RA was significantly lower in the presence of the C allele for 6365T-->C (OR 0.50, 95% CI 0.29-0.85, in a recessive model, and OR 0.80, 95% CI 0.67-0.97, in a codominant model). The C allele for -899G-->C was also associated with a significantly lower risk of RA (OR 0.67, 95% CI 0.48-0.95, in a codominant model). The radiologic severity of RA was not associated with COX-2 polymorphisms. CONCLUSION: Our study revealed a possible protective influence of the C allele for 6365T-->C and for -899G-->C in RA. ER -