@article {Roelofs558, author = {Mieke F Roelofs and Mark H Wenink and Erik J M Toonen and Marieke J H Coenen and Leo A B Joosten and Wim B van den Berg and Piet L C M van Riel and Timothy R D J Radstake}, title = {The functional variant (Asp299gly) of toll-like receptor 4 (TLR4) influences TLR4-mediated cytokine production in rheumatoid arthritis.}, volume = {35}, number = {4}, pages = {558--561}, year = {2008}, publisher = {The Journal of Rheumatology}, abstract = {OBJECTIVE: To investigate functional consequences of the Toll-like receptor 4 (TLR4) variant (Asp299Gly) in rheumatoid arthritis (RA). METHODS: Peripheral blood mononuclear cells from 28 patients with RA carrying or not carrying the TLR4 variant were incubated with lipopolysaccharide (LPS) and heat shock protein B8 (HSPB8). Concentrations of interleukin 6 (IL-6), tumor necrosis factor-alpha(TNF-alpha), and IL-10 were determined along with TLR4 and CD14 expression. RESULTS: TLR4 expression was similar in patients carrying or not carrying the variant. In contrast, both LPS and HSPB8 resulted in significantly lower secretion of IL-6, TNF-alpha, and IL-10 in those who carried the variant, whereas the frequency of CD14+ cells was higher in these individuals. CONCLUSION: TLR4 variant clearly reduces its potency to mediate signaling. Correction for CD14+ cells is necessary in comparable experiments.}, issn = {0315-162X}, URL = {https://www.jrheum.org/content/35/4/558}, eprint = {https://www.jrheum.org/content/35/4/558.full.pdf}, journal = {The Journal of Rheumatology} }