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Research ArticleArticle

Role of MiR-98 and Its Underlying Mechanisms in Systemic Lupus Erythematosus

Lin Xie and Jinhua Xu
The Journal of Rheumatology June 2018, jrheum.171290; DOI: https://doi.org/10.3899/jrheum.171290
Lin Xie
From the Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China. Supported by grants from the National Natural Science Foundation of China (No. 81373212) and from Shanghai Municipal Commission of Health and Family Planning (No. 2017ZZ02002). L. Xie, MD, PhD; J. Xu, MD, PhD, Professor, Department of Dermatology, Huashan Hospital, Fudan University. Address correspondence to Dr. J. Xu, Department of Dermatology, Huashan Hospital, Fudan University, No. 12 Middle Wulumuqi Road, Shanghai 200040, China. E-mail: xjhhsyy@163.com. Accepted for publication April 30, 2018.
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Jinhua Xu
From the Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, China. Supported by grants from the National Natural Science Foundation of China (No. 81373212) and from Shanghai Municipal Commission of Health and Family Planning (No. 2017ZZ02002). L. Xie, MD, PhD; J. Xu, MD, PhD, Professor, Department of Dermatology, Huashan Hospital, Fudan University. Address correspondence to Dr. J. Xu, Department of Dermatology, Huashan Hospital, Fudan University, No. 12 Middle Wulumuqi Road, Shanghai 200040, China. E-mail: xjhhsyy@163.com. Accepted for publication April 30, 2018.
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Abstract

Objective T-lymphocyte apoptosis plays a critical role in the pathogenesis of systemic lupus erythematosus (SLE). However, the underlying regulatory mechanisms of apoptosis in SLE remain unclear. The aim of this study was to explore the role of miR-98 in SLE and its underlying mechanisms.

Methods Western blotting and quantitative reverse transcription PCR (qRT-PCR) were used to analyze miR-98 and Fas expression. Luciferase reporter assays were performed to identify miR-98 targets. To modify miRNA levels, miR-98 mimics and inhibitor were transfected into cells. A lentiviral construct was used to overexpress the level of Fas in SLE CD4+ T cells. Gene and protein expression were determined by qRT-PCR and Western blotting. Apoptosis levels were evaluated by annexin V staining and flow cytometry.

Results Compared to those of healthy donors, miR-98 was downregulated in SLE CD4+ T cells, whereas Fas mRNA and protein expression were upregulated. Upregulation of miR-98 by mimic transfection protected Jurkat cells against Fas-mediated apoptosis at both mRNA and protein levels, while miR-98 inhibitor induced the completely opposite effect. Luciferase reporter assays demonstrated that miR-98 directly targeted Fas mRNA. Further, miR-98 inhibitor induced apoptosis in primary healthy CD4+ T cells through the Fas-caspase axis, while upregulation of miR-98 in SLE CD4+ T cells led to the opposite effect.

Conclusion The current study revealed that downregulation of miR-98 induces apoptosis by modulating the Fas-mediated apoptotic signaling pathway in SLE CD4+ T cells. These results suggest that miR-98 might serve as a potential target for SLE treatment.

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The Journal of Rheumatology: 46 (2)
The Journal of Rheumatology
Vol. 46, Issue 2
1 Feb 2019
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Role of MiR-98 and Its Underlying Mechanisms in Systemic Lupus Erythematosus
Lin Xie, Jinhua Xu
The Journal of Rheumatology Jun 2018, jrheum.171290; DOI: 10.3899/jrheum.171290

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Role of MiR-98 and Its Underlying Mechanisms in Systemic Lupus Erythematosus
Lin Xie, Jinhua Xu
The Journal of Rheumatology Jun 2018, jrheum.171290; DOI: 10.3899/jrheum.171290
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