Abstract
Objective. To investigate the role of thrombospondin 1 (TSP-1) in RA.
Methods. Expression of TSP-1 in synovial tissues was determined by immunohistochemistry. Expression of TSP-1 in rheumatoid fibroblast-like synovial cells (FLS) was investigated by quantitative real-time PCR and ELISA. Correlations among the plasma TSP-1 and other variables in patients with RA were examined.
Results. Expression of TSP-1 was increased in rheumatoid synovial tissues. Transforming growth factor-β1 (TGF-β1) clearly increased TSP-1 expression in FLS on both mRNA and protein levels. Changes in plasma TSP-1 were associated with those in 28-joint Disease Activity Score-erythrocyte sedimentation rate and plasma TGF-β1.
Conclusion. TSP-1 might be critically involved in the disease process of RA through the TGF-β1/TSP-1 axis.
- RHEUMATOID ARTHRITIS
- FIBROBLAST-LIKE SYNOVIAL CELL
- SYNOVITIS
- THROMBOSPONDIN 1
- TRANSFORMING GROWTH FACTOR-Β1
- BIOMARKER
Footnotes
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Supported in part by a grant from the Ministry of Health, Labor, and Welfare, Japan.
- Accepted for publication February 24, 2015.